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- W8158766 abstract "Enzyme-linked immunosorbent assay (ELISA) for assaying the 5-enolpyruvyshikimate-3-phosphate synthase from Agribacterium sp. CP4 (CP4 EPSPS) in genetically modified soybeans was developed. Polyclonal and monoclonal antibodies (Pab, Mab) specific to the CP4 EPSPS were produced. When using the Pab, the detection limit of sandwich ELISA toward CP4 EPSPS (0.03 ) was better than that of competitive indirect ELISA(ciELISA) (1 ). It was found that 2 of 3 monoclonal antibodies, Mab1 and Mab2, recognized the same antigenic determinant on CP4 EPSPS, but Mab3 recognized different antigenic determinant when competitive ELISA was performed using the Mabs. On the other hand, when the sensitivity of sandwich ELISA using combination of Pab and/or Mabs was determined, the sandiwich ELISA using Mab2 as a capture antibody and Pab-HRP as a secondary antibody showed the lowest detection limit of CP4 EPSPS (0.02 ). The sandwich ELISA developed in this study could be applied to detect glyphosate-tolerant soybeans." @default.
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- W8158766 date "2003-01-01" @default.
- W8158766 modified "2023-09-23" @default.
- W8158766 title "Development of Enzyme-Linked Immunosorbent Assay for Glyphosate-Tolerant Soybeans" @default.
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