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• W81590852 abstract "Research Article1 June 1989free access Epstein-Barr virus bicistronic mRNAs generated by facultative splicing code for two transcriptional trans-activators. E. Manet E. Manet Laboratoire de Virologie Moléculaire, Ecole Normale Supérieure de Lyon, CNRS (UMR13), France. Search for more papers by this author H. Gruffat H. Gruffat Laboratoire de Virologie Moléculaire, Ecole Normale Supérieure de Lyon, CNRS (UMR13), France. Search for more papers by this author M. C. Trescol-Biemont M. C. Trescol-Biemont Laboratoire de Virologie Moléculaire, Ecole Normale Supérieure de Lyon, CNRS (UMR13), France. Search for more papers by this author N. Moreno N. Moreno Laboratoire de Virologie Moléculaire, Ecole Normale Supérieure de Lyon, CNRS (UMR13), France. Search for more papers by this author P. Chambard P. Chambard Laboratoire de Virologie Moléculaire, Ecole Normale Supérieure de Lyon, CNRS (UMR13), France. Search for more papers by this author J. F. Giot J. F. Giot Laboratoire de Virologie Moléculaire, Ecole Normale Supérieure de Lyon, CNRS (UMR13), France. Search for more papers by this author A. Sergeant A. Sergeant Laboratoire de Virologie Moléculaire, Ecole Normale Supérieure de Lyon, CNRS (UMR13), France. Search for more papers by this author E. Manet E. Manet Laboratoire de Virologie Moléculaire, Ecole Normale Supérieure de Lyon, CNRS (UMR13), France. Search for more papers by this author H. Gruffat H. Gruffat Laboratoire de Virologie Moléculaire, Ecole Normale Supérieure de Lyon, CNRS (UMR13), France. Search for more papers by this author M. C. Trescol-Biemont M. C. Trescol-Biemont Laboratoire de Virologie Moléculaire, Ecole Normale Supérieure de Lyon, CNRS (UMR13), France. Search for more papers by this author N. Moreno N. Moreno Laboratoire de Virologie Moléculaire, Ecole Normale Supérieure de Lyon, CNRS (UMR13), France. Search for more papers by this author P. Chambard P. Chambard Laboratoire de Virologie Moléculaire, Ecole Normale Supérieure de Lyon, CNRS (UMR13), France. Search for more papers by this author J. F. Giot J. F. Giot Laboratoire de Virologie Moléculaire, Ecole Normale Supérieure de Lyon, CNRS (UMR13), France. Search for more papers by this author A. Sergeant A. Sergeant Laboratoire de Virologie Moléculaire, Ecole Normale Supérieure de Lyon, CNRS (UMR13), France. Search for more papers by this author Author Information E. Manet1, H. Gruffat1, M. C. Trescol-Biemont1, N. Moreno1, P. Chambard1, J. F. Giot1 and A. Sergeant1 1Laboratoire de Virologie Moléculaire, Ecole Normale Supérieure de Lyon, CNRS (UMR13), France. The EMBO Journal (1989)8:1819-1826https://doi.org/10.1002/j.1460-2075.1989.tb03576.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The Epstein-Barr virus (EBV) genome codes for several transcriptional trans-activators. One of them, the BZLF1 open reading frame (ORF)-encoded product EB1, is able to induce the productive cycle in infected B cells. From the cloning and characterization of full-length cDNAs, we found that EB1 could be made from three overlapping messenger RNAs expressed under the control of two different promoters that we call P1 and P2. The first mRNA, 1 kb long, is made from the P1 promoter and codes for EB1 alone. The two other mRNAs, respectively 3 and 4 kb long and made by facultative splicing, are bicistronic mRNAs. They code not only for the trans-activator EB1 but also for a second EBV transcriptional trans-activator R, encoded by the BRLF1 ORF. In effect, authentic EB1 and R proteins are expressed from the 3 and 4 kb long cDNAs as demonstrated by identification of the proteins with specific antisera. In addition, EB1 and R expressed from the 3 and 4 kb cDNAs activate transcription from their specific targets in the EBV early promoter DR. Previous ArticleNext Article Volume 8Issue 61 June 1989In this issue RelatedDetailsLoading ..." @default.
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• W81590852 title "Epstein-Barr virus bicistronic mRNAs generated by facultative splicing code for two transcriptional trans-activators." @default.
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