FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W817440729> ?p ?o ?g. }

• W817440729 abstract "Since the 1970?s there has been industrial interest in using microorganisms as microcapsules. The encapsulation of actives (e.g. flavours, drugs, perfumes) is a necessary process for pharmaceutical and food companies because the precious and often expensive ingredients must be protected from degradation and also released in a specific site or under a specific stimulus. Saccharomyces cerevisiae, baker?s yeast, represents a first choice microorganism for the encapsulation of active ingredients. It is biodegradable and biocompatible with human digestion and skin, and can be produced in an easy and cheap way. A major part of this project has been dedicated to the development of robust methods of extraction and quantification of hydrophobic substances loaded inside yeast cells, which have been subsequently combined with an indirect, fluorescence-based method for the evaluation of the rate of loading of hydrophobic substances in the same cells. In particular, it has been found that this process reaches a limit in the maximal loading capacity of intact yeast cells, most likely reflecting the maximal volume of the lipid droplet organelles in which loaded hydrophobes accumulate. With the new on-line (fluorescence-based) and off-line (chromatography-based) methods developed here it has been established that the loading process fundamentally follows a diffusion model, in which the solubility in water determines the permeation of substances through the cell wall and ultimately their uptake by yeast cells. However, treating yeast cells with organic solvents like DMSO - a new approach introduced in Prof. Tirelli?s lab to enhance the encapsulation of hydrophobes ? completely changes the chemical-physical parameters of the encapsulation process. In DMSO-treated cells, substances are loaded fundamentally in response to their hydrophobicity. Conversely, once loaded, the same substances are released with a rate that is inversely proportional to their hydrophobicity, as observed by applying a novel approach to measure the release of hydrophobes encapsulated in yeast cells, either in the absence of presence of DMSO-treatment. In conclusion, the new evidence reported here clarifies basic aspects of hydrophobe encapsulation in intact yeast cells and will thus help improving future applications of these microcapsules as a valid, inexpensive and biocompatible drug delivery system." @default.
• W817440729 created "2016-06-24" @default.
• W817440729 creator A5062855451 @default.
• W817440729 date "2011-09-17" @default.
• W817440729 modified "2023-09-23" @default.
• W817440729 title "Characterisation of microencapsulation process in Saccharomyces cerevisiae" @default.
• W817440729 hasPublicationYear "2011" @default.
• W817440729 type Work @default.
• W817440729 sameAs 817440729 @default.
• W817440729 citedByCount "0" @default.
• W817440729 crossrefType "dissertation" @default.
• W817440729 hasAuthorship W817440729A5062855451 @default.
• W817440729 hasConcept C155574463 @default.
• W817440729 hasConcept C178790620 @default.
• W817440729 hasConcept C185592680 @default.
• W817440729 hasConcept C2777576037 @default.
• W817440729 hasConcept C2779222958 @default.
• W817440729 hasConcept C41625074 @default.
• W817440729 hasConcept C43617362 @default.
• W817440729 hasConcept C50670333 @default.
• W817440729 hasConcept C55493867 @default.
• W817440729 hasConceptScore W817440729C155574463 @default.
• W817440729 hasConceptScore W817440729C178790620 @default.
• W817440729 hasConceptScore W817440729C185592680 @default.
• W817440729 hasConceptScore W817440729C2777576037 @default.
• W817440729 hasConceptScore W817440729C2779222958 @default.
• W817440729 hasConceptScore W817440729C41625074 @default.
• W817440729 hasConceptScore W817440729C43617362 @default.
• W817440729 hasConceptScore W817440729C50670333 @default.
• W817440729 hasConceptScore W817440729C55493867 @default.
• W817440729 hasLocation W8174407291 @default.
• W817440729 hasOpenAccess W817440729 @default.
• W817440729 hasPrimaryLocation W8174407291 @default.
• W817440729 hasRelatedWork W1036868670 @default.
• W817440729 hasRelatedWork W1177100998 @default.
• W817440729 hasRelatedWork W123575851 @default.
• W817440729 hasRelatedWork W1510225169 @default.
• W817440729 hasRelatedWork W168963999 @default.
• W817440729 hasRelatedWork W1990330500 @default.
• W817440729 hasRelatedWork W1994203434 @default.
• W817440729 hasRelatedWork W1999633306 @default.
• W817440729 hasRelatedWork W2120424690 @default.
• W817440729 hasRelatedWork W2157029600 @default.
• W817440729 hasRelatedWork W2329875767 @default.
• W817440729 hasRelatedWork W2489959234 @default.
• W817440729 hasRelatedWork W2956099672 @default.
• W817440729 hasRelatedWork W3049617231 @default.
• W817440729 hasRelatedWork W976280820 @default.
• W817440729 hasRelatedWork W1824760253 @default.
• W817440729 hasRelatedWork W2098309756 @default.
• W817440729 hasRelatedWork W2549807416 @default.
• W817440729 hasRelatedWork W2772360935 @default.
• W817440729 hasRelatedWork W2965224929 @default.
• W817440729 isParatext "false" @default.
• W817440729 isRetracted "false" @default.
• W817440729 magId "817440729" @default.
• W817440729 workType "dissertation" @default.