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• W81757823 abstract "Loop-mediated isothermal amplification (LAMP) is a rapid and reliable sequence-specific isothermal nucleic acid amplification technique. To date, all reported real-time detection methods for LAMP have been restricted to single targets, limiting the utility of this technique. Here, we adapted standard LAMP primers to contain a quencher-fluorophore duplex region that upon strand separation results in a gain of fluorescent signal. This approach permitted the real-time detection of 1–4 target sequences in a single LAMP reaction tube utilizing a standard real-time fluorimeter. The methodology was highly reproducible and sensitive, detecting below 100 copies of human genomic DNA. It was also robust, with a 7-order of magnitude dynamic range of detectable targets. Furthermore, using a new strand-displacing DNA polymerase or its warm-start version, Bst 2.0 or Bst 2.0 WarmStart DNA polymerases, resulted in 50% faster amplification signals than wild-type Bst DNA polymerase, large fragment in this new multiplex LAMP procedure. The coupling of this new multiplex technique with next generation isothermal DNA polymerases should increase the utility of the LAMP method for molecular diagnostics." @default.
• W81757823 created "2016-06-24" @default.
• W81757823 creator A5034315022 @default.
• W81757823 creator A5035914775 @default.
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• W81757823 date "2012-08-01" @default.
• W81757823 modified "2023-10-10" @default.
• W81757823 title "Simultaneous multiple target detection in real-time loop-mediated isothermal amplification" @default.
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• W81757823 doi "https://doi.org/10.2144/0000113902" @default.
• W81757823 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23030060" @default.
• W81757823 hasPublicationYear "2012" @default.
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