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• W818254209 abstract "The Bordetella pertussis gene encoding superoxide dismutase (SOD), termed sodB, was cloned. The nucleotide sequence of sodB predicted a 21-kDa protein with homology to iron-containing SODs. Examination of SOD activity gels suggests that B. pertussis extracts contain a single SOD that utilizes Fe3+ as its prosthetic group. A SODdeficient mutant, DD25, was obtained by insertional inactivation of sodB in B. pertussis. DD25 was more sensitive to killing by the superoxide-generating compound paraquat than the parental strain BP339. Both strains survived equally in human polymorphonuclear leukocytes (PMN). Crude extracts of B. pertussis contain a single catalase. B. pertussis katA was cloned and the nucleotide sequence predicted a 55-kDa protein that shared homology with heme-containing catalases. Analysis of the nucleotide sequence upstream of katA revealed the presence of a copy of IS481, a B. pertussis-specific insertion sequence. The start site of transcription of katA was mapped to a T residue in IS481 by primer extension analysis. A catalase-deficient strain of B. pertussis, DD9oo, was constructed and was more sensitive to H202 killing than the parental strain BP339. However, there was no difference in the ability of either strain to survive in PMN. The genes encoding pertussis toxin (ptx) and adenylate cyclase toxin (cya) are positively regulated in B. pertussis by the bvgAS locus. However, ptx::lacZ and cya::lacZ transcriptional fusions in E. coli cannot be activated by bvgAS in trans. This suggests that an additional factor(s) is required for transcription of these genes. A gene encoding a Bvg accessory factor (BAF) was identified by it's ability to activate plx::lacZ in the presence of bvgAS. The expression of ptx::lacZ was decreased by the addition of 40 mM MgS04 , a compound that also modulates pIX expression in B. pertussis. An additional copy of baf was integrated into the chromosome of BC75, a B. pertussis mutant that expresses low levels of pIX and cya. The defect in BC75 was partially" @default.
• W818254209 created "2016-06-24" @default.
• W818254209 creator A5000934588 @default.
• W818254209 date "1994-01-01" @default.
• W818254209 modified "2023-09-24" @default.
• W818254209 title "Genetics of virulence in Bordetella pertussis. I. Characterization of genes for superoxide dismutase and catalase. II. Identification of a new putative transcriptional regulator." @default.
• W818254209 cites W1022042346 @default.
• W818254209 cites W1487254759 @default.
• W818254209 cites W1501994989 @default.
• W818254209 cites W1509038183 @default.
• W818254209 cites W1511640438 @default.
• W818254209 cites W1512520069 @default.
• W818254209 cites W1546783991 @default.
• W818254209 cites W1551413691 @default.
• W818254209 cites W1556957447 @default.
• W818254209 cites W1566044277 @default.
• W818254209 cites W1579263673 @default.
• W818254209 cites W1597468158 @default.
• W818254209 cites W1602739040 @default.
• W818254209 cites W1606784082 @default.
• W818254209 cites W1622600192 @default.
• W818254209 cites W1690864707 @default.
• W818254209 cites W1698425754 @default.
• W818254209 cites W1745436806 @default.
• W818254209 cites W1782573390 @default.
• W818254209 cites W179059320 @default.
• W818254209 cites W1814215520 @default.
• W818254209 cites W1819368333 @default.
• W818254209 cites W1827240104 @default.
• W818254209 cites W1838165025 @default.
• W818254209 cites W1847681443 @default.
• W818254209 cites W1850763480 @default.
• W818254209 cites W1860316592 @default.
• W818254209 cites W1866769789 @default.
• W818254209 cites W186907087 @default.
• W818254209 cites W1878981575 @default.
• W818254209 cites W1890653201 @default.
• W818254209 cites W1900978828 @default.
• W818254209 cites W1903871422 @default.
• W818254209 cites W1910268468 @default.
• W818254209 cites W191793640 @default.
• W818254209 cites W1927634469 @default.
• W818254209 cites W1932302518 @default.
• W818254209 cites W1939644720 @default.
• W818254209 cites W1940436875 @default.
• W818254209 cites W1944496202 @default.
• W818254209 cites W1946864347 @default.
• W818254209 cites W1947132191 @default.
• W818254209 cites W1947308452 @default.
• W818254209 cites W1947905148 @default.
• W818254209 cites W1951572341 @default.
• W818254209 cites W1962485516 @default.
• W818254209 cites W1963722963 @default.
• W818254209 cites W1970864527 @default.
• W818254209 cites W1972673475 @default.
• W818254209 cites W1975085668 @default.
• W818254209 cites W1975875982 @default.
• W818254209 cites W1975998142 @default.
• W818254209 cites W1977415113 @default.
• W818254209 cites W1978274173 @default.
• W818254209 cites W1980011555 @default.
• W818254209 cites W1981104297 @default.
• W818254209 cites W1982795035 @default.
• W818254209 cites W1989725152 @default.
• W818254209 cites W1993096337 @default.
• W818254209 cites W1996176419 @default.
• W818254209 cites W1997481265 @default.
• W818254209 cites W2000055267 @default.
• W818254209 cites W2000559951 @default.
• W818254209 cites W2001249476 @default.
• W818254209 cites W2001312077 @default.
• W818254209 cites W2007457312 @default.
• W818254209 cites W2008103563 @default.
• W818254209 cites W2011521909 @default.
• W818254209 cites W2011536718 @default.
• W818254209 cites W2012191122 @default.
• W818254209 cites W2013537559 @default.
• W818254209 cites W2017365299 @default.
• W818254209 cites W2024596310 @default.
• W818254209 cites W2027229485 @default.
• W818254209 cites W2028622989 @default.
• W818254209 cites W2028714053 @default.
• W818254209 cites W2029514347 @default.
• W818254209 cites W2035678345 @default.
• W818254209 cites W2038509945 @default.
• W818254209 cites W2039184617 @default.
• W818254209 cites W2040084758 @default.
• W818254209 cites W2045203765 @default.
• W818254209 cites W2047544081 @default.
• W818254209 cites W2048895914 @default.
• W818254209 cites W2049338606 @default.
• W818254209 cites W2052510558 @default.
• W818254209 cites W2052545305 @default.
• W818254209 cites W2054020755 @default.
• W818254209 cites W2054267584 @default.
• W818254209 cites W2055622788 @default.
• W818254209 cites W2056074494 @default.
• W818254209 cites W2056168344 @default.
• W818254209 cites W2056613668 @default.
• W818254209 cites W2058007274 @default.

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