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• W818657967 abstract "Background: Artificial inseminations in swine are conducted with liquid semen cooled at 15 to 18oC for 1 to 5 d. Frozen semen in not routinely used due to its poor reproductive performance in comparison with cooled semen. In semen freezing protocols, egg yolk is added to extenders to protect the sperm membrane against cold shock. The cryoprotectant effect of egg yolk is attributed to the presence of low density lipoprotein (LDL) in its composition. Thus, replacement of egg yolk by LDL in the composition of extenders may be feasible to reduce cryoinjuries in sperm cells due to cold shock. During the process of sperm freezing the cell receive structural and functional injuries that could impair the fertilization process. Two experiments were conducted to evaluate the effect of freezing method, extenders and duration of the freezing process. Materials, Methods and Results: In experiment 1 post-thawing motility of the Westendorf (WE) method was higher (P 0.05) in membrane integrity after thawing between Short (40.3±2.4) and Standard (38.7±2.8) methods. The analysis was performed using the software SAS. Discussion: Sperm motility and membrane integrity were greater for the WE method than for the PA method, which can be due to the presence of seminal plasma during cooling, since the benefits of the presence of seminal plasma proteins have been widely reported. In the PA method, the glycerol-based freezing extender was added to semen at 15oC, remaining in contact with the semen for 60 min before freezing. On the other hand, in the WE method, the glycerol-based freezing extender was added to semen only at the temperature of 5oC. Thus, the reduced sperm motility and membrane integrity observe in the PA method may have occurred as a function of the prolonged exposure to glycerol. The presence of a non-penetrating cryoprotectant (either egg yolk or LDL) during cooling at temperatures greater than 15oC did not benefit sperm motility and membrane integrity. That may have been due to the low content of both egg yolk and LDL added to the extender (2%) which may not been enough to ensure cryoprotection. The 120m cooling period up to 15oC was efficient on stabilizing the sperm cells in the extenders, protecting then against cold shock, which allowed a substantial reduction in the period necessary to complete the freezing process. The use of WE method associated BTS extender and Short freezing method was the most efficient protocol" @default.
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• W818657967 date "2011-01-01" @default.
• W818657967 modified "2023-09-24" @default.
• W818657967 title "Effect of different freezing methods, extenders and duration of freezing on quality of frozen boar semen." @default.
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