Matches in SemOpenAlex for { <https://semopenalex.org/work/W819965820> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W819965820 endingPage "118" @default.
- W819965820 startingPage "113" @default.
- W819965820 abstract "Among a number of antigens characterized in M leprae, an etiological agent of Leprosy, the 18 kDa antigen, is unique to M leprae. We have previously determined a sequence specific element in the 18 kDa gene of M leprae, which confers transcriptional repression. In this report, we have examined if the element could be applied to genes other than the 18 kDa gene of M leprae. To identify the roles of the regulatory sequence in heterologous promoter, we have constructed pB3 vector series, which contains BCG hsp65 promoter and the M leprae 18 kDa transcription repression responsive element in tandem using LacZ gene as a reporter gene. Cloning of hsp65 promoters of M bovis BCG or M smegmatis in front of LacZ gene resulted in normal galactosidase activity as expected. However, when the sequence element was placed between the promoter and the LacZ gene, -galactosidase activity was reduced 10-fold less. Also we have examined with pB3(-) vector, that harbors the transcription repression responsive element in a reversed orientation, the -galactosidase activity was found to be similar to pB3(+) vector. Thus, these results further confirm that M leprae 18 kDa transcription repression responsive element could regulate BCG hsp65 heterologous promoter and that the element could act as an operator for the transcription of mycobacteria." @default.
- W819965820 created "2016-06-24" @default.
- W819965820 creator A5008640196 @default.
- W819965820 creator A5089597641 @default.
- W819965820 date "2003-01-01" @default.
- W819965820 modified "2023-09-24" @default.
- W819965820 title "Heterologous Regulation of BCG hsp65 Promoter by M.leprae 18 kDa Transcription Repression Responsive Element" @default.
- W819965820 hasPublicationYear "2003" @default.
- W819965820 type Work @default.
- W819965820 sameAs 819965820 @default.
- W819965820 citedByCount "0" @default.
- W819965820 crossrefType "journal-article" @default.
- W819965820 hasAuthorship W819965820A5008640196 @default.
- W819965820 hasAuthorship W819965820A5089597641 @default.
- W819965820 hasConcept C101762097 @default.
- W819965820 hasConcept C104317684 @default.
- W819965820 hasConcept C132376346 @default.
- W819965820 hasConcept C138885662 @default.
- W819965820 hasConcept C150194340 @default.
- W819965820 hasConcept C153911025 @default.
- W819965820 hasConcept C158448853 @default.
- W819965820 hasConcept C161733203 @default.
- W819965820 hasConcept C170344550 @default.
- W819965820 hasConcept C179926584 @default.
- W819965820 hasConcept C186310378 @default.
- W819965820 hasConcept C22744801 @default.
- W819965820 hasConcept C2780115692 @default.
- W819965820 hasConcept C41895202 @default.
- W819965820 hasConcept C54355233 @default.
- W819965820 hasConcept C86339819 @default.
- W819965820 hasConcept C86803240 @default.
- W819965820 hasConceptScore W819965820C101762097 @default.
- W819965820 hasConceptScore W819965820C104317684 @default.
- W819965820 hasConceptScore W819965820C132376346 @default.
- W819965820 hasConceptScore W819965820C138885662 @default.
- W819965820 hasConceptScore W819965820C150194340 @default.
- W819965820 hasConceptScore W819965820C153911025 @default.
- W819965820 hasConceptScore W819965820C158448853 @default.
- W819965820 hasConceptScore W819965820C161733203 @default.
- W819965820 hasConceptScore W819965820C170344550 @default.
- W819965820 hasConceptScore W819965820C179926584 @default.
- W819965820 hasConceptScore W819965820C186310378 @default.
- W819965820 hasConceptScore W819965820C22744801 @default.
- W819965820 hasConceptScore W819965820C2780115692 @default.
- W819965820 hasConceptScore W819965820C41895202 @default.
- W819965820 hasConceptScore W819965820C54355233 @default.
- W819965820 hasConceptScore W819965820C86339819 @default.
- W819965820 hasConceptScore W819965820C86803240 @default.
- W819965820 hasIssue "2" @default.
- W819965820 hasLocation W8199658201 @default.
- W819965820 hasOpenAccess W819965820 @default.
- W819965820 hasPrimaryLocation W8199658201 @default.
- W819965820 hasRelatedWork W121607532 @default.
- W819965820 hasRelatedWork W1570879822 @default.
- W819965820 hasRelatedWork W1581805224 @default.
- W819965820 hasRelatedWork W1979993901 @default.
- W819965820 hasRelatedWork W1986476375 @default.
- W819965820 hasRelatedWork W1988996371 @default.
- W819965820 hasRelatedWork W1996843168 @default.
- W819965820 hasRelatedWork W2002659630 @default.
- W819965820 hasRelatedWork W2036577672 @default.
- W819965820 hasRelatedWork W2057328972 @default.
- W819965820 hasRelatedWork W2068055075 @default.
- W819965820 hasRelatedWork W2085376195 @default.
- W819965820 hasRelatedWork W2115342123 @default.
- W819965820 hasRelatedWork W2116433409 @default.
- W819965820 hasRelatedWork W2133645968 @default.
- W819965820 hasRelatedWork W2136098448 @default.
- W819965820 hasRelatedWork W2156853081 @default.
- W819965820 hasRelatedWork W2390807259 @default.
- W819965820 hasRelatedWork W3203880995 @default.
- W819965820 hasRelatedWork W2508630470 @default.
- W819965820 hasVolume "1" @default.
- W819965820 isParatext "false" @default.
- W819965820 isRetracted "false" @default.
- W819965820 magId "819965820" @default.
- W819965820 workType "article" @default.