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- W82029207 abstract "Research Article1 May 1986free access The use of incomplete genes for the construction of a Trypanosoma equiperdum variant surface glycoprotein gene. C.W. Roth C.W. Roth Search for more papers by this author S. Longacre S. Longacre Search for more papers by this author A. Raibaud A. Raibaud Search for more papers by this author T. Baltz T. Baltz Search for more papers by this author H. Eisen H. Eisen Search for more papers by this author C.W. Roth C.W. Roth Search for more papers by this author S. Longacre S. Longacre Search for more papers by this author A. Raibaud A. Raibaud Search for more papers by this author T. Baltz T. Baltz Search for more papers by this author H. Eisen H. Eisen Search for more papers by this author Author Information C.W. Roth, S. Longacre, A. Raibaud, T. Baltz and H. Eisen The EMBO Journal (1986)5:1065-1070https://doi.org/10.1002/j.1460-2075.1986.tb04323.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The expression of Trypanosoma equiperdum variant surface protein (VSG) 78 is accomplished by the duplicative transposition of silent basic copy (BC) genes into a telomer-linked expression site to form an expression-linked copy (ELC). In two independent isolates expressing VSG 78, the ELC is a composite gene. The analysis of VSG 78 cDNA clones from these two Bo Tat 78 isolates and the respective BC genes revealed that both ELCs were constructed from the same three BC genes, a 3′ BC which donated the last 255 bp of each ELC and two closely related 5′ BCs. Although sequences of both 5′ BC genes were found in each ELC, the junction with the 3′ BC was provided by the same 5′ BC in both cases. This 5′ BC is an incomplete gene with insufficient open reading frame to code for a complete VSG and thus can only be used when joined to a competent 3′ end. Furthermore, both 5′ BC genes lack a conserved 14 nucleotide sequence found on all VSG mRNAs. These results support a model in which composite gene formation plays a role in the determination of the order of VSG expression. They also illustrate similarities between immunoglobulin gene and VSG gene construction. Previous ArticleNext Article Volume 5Issue 51 May 1986In this issue RelatedDetailsLoading ..." @default.
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- W82029207 title "The use of incomplete genes for the construction of a Trypanosoma equiperdum variant surface glycoprotein gene." @default.
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