FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W82168767> ?p ?o ?g. }

• W82168767 endingPage "1484" @default.
• W82168767 startingPage "1479" @default.
• W82168767 abstract "Research Article1 May 1989free access Nuclear transport kinetics depend on phosphorylation-site-containing sequences flanking the karyophilic signal of the Simian virus 40 T-antigen. H. P. Rihs H. P. Rihs Max-Planck-Institut für Biophysik, Frankfurt, FRG. Search for more papers by this author R. Peters R. Peters Max-Planck-Institut für Biophysik, Frankfurt, FRG. Search for more papers by this author H. P. Rihs H. P. Rihs Max-Planck-Institut für Biophysik, Frankfurt, FRG. Search for more papers by this author R. Peters R. Peters Max-Planck-Institut für Biophysik, Frankfurt, FRG. Search for more papers by this author Author Information H. P. Rihs1 and R. Peters1 1Max-Planck-Institut für Biophysik, Frankfurt, FRG. The EMBO Journal (1989)8:1479-1484https://doi.org/10.1002/j.1460-2075.1989.tb03531.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Selective nuclear protein transport was analyzed in single living cells. Hybrid proteins consisting of short stretches of the Simian virus 40 T-antigen and of the almost complete beta-galactosidase moiety were generated by molecular genetic methods and injected into the cytoplasm of rodent hepatoma cells. A histochemical assay showed that all proteins containing the karyophilic signal of the T-antigen (residues 126/127-132) were equally well accumulated by the nucleus within 15 h after injection. Microfluorimetric measurements of nuclear transport kinetics, however, revealed large differences. Proteins containing the karyophilic signal without flanking sequences were taken up by the nucleus on a time scale of hours. The same held for a protein containing T-antigen residues 127-147. However, a protein containing T-antigen residues 111-135 was accumulated by the nucleus with a half-time of 8-10 min reaching an equilibrium nucleocytoplasmic concentration ratio of greater than or equal to 15. Photobleaching measurements showed that, independently of subcellular localization, the mobility of all proteins was quite large. Thus, our measurements revealed a striking effect of T-antigen residues 111-125 on the kinetics of nuclear transport. Residues 111-125 do not seem to contain a second karyophilic signal. Conspicuously, however, they comprise a cluster of phosphorylation sites. Previous ArticleNext Article Volume 8Issue 51 May 1989In this issue RelatedDetailsLoading ..." @default.
• W82168767 created "2016-06-24" @default.
• W82168767 creator A5041664877 @default.
• W82168767 creator A5067382234 @default.
• W82168767 date "1989-05-01" @default.
• W82168767 modified "2023-10-15" @default.
• W82168767 title "Nuclear transport kinetics depend on phosphorylation-site-containing sequences flanking the karyophilic signal of the Simian virus 40 T-antigen." @default.
• W82168767 cites W119651583 @default.
• W82168767 cites W1512281481 @default.
• W82168767 cites W1535454837 @default.
• W82168767 cites W1549525782 @default.
• W82168767 cites W175043000 @default.
• W82168767 cites W1858391321 @default.
• W82168767 cites W1892601883 @default.
• W82168767 cites W1965609818 @default.
• W82168767 cites W1975589498 @default.
• W82168767 cites W1981943850 @default.
• W82168767 cites W1989308870 @default.
• W82168767 cites W1991486518 @default.
• W82168767 cites W2001111968 @default.
• W82168767 cites W2007121427 @default.
• W82168767 cites W2021735119 @default.
• W82168767 cites W2023266365 @default.
• W82168767 cites W2025350460 @default.
• W82168767 cites W2028622989 @default.
• W82168767 cites W2033722975 @default.
• W82168767 cites W2035609763 @default.
• W82168767 cites W2041389127 @default.
• W82168767 cites W2046645183 @default.
• W82168767 cites W2051847700 @default.
• W82168767 cites W2053796273 @default.
• W82168767 cites W2077213761 @default.
• W82168767 cites W2080575639 @default.
• W82168767 cites W2084800523 @default.
• W82168767 cites W2092084168 @default.
• W82168767 cites W2118608275 @default.
• W82168767 cites W2130147634 @default.
• W82168767 cites W2133531135 @default.
• W82168767 cites W2138270253 @default.
• W82168767 cites W2141529118 @default.
• W82168767 cites W2143863925 @default.
• W82168767 cites W2147716555 @default.
• W82168767 cites W2151273144 @default.
• W82168767 cites W2163400869 @default.
• W82168767 cites W2173844423 @default.
• W82168767 cites W4234462880 @default.
• W82168767 cites W892675331 @default.
• W82168767 doi "https://doi.org/10.1002/j.1460-2075.1989.tb03531.x" @default.
• W82168767 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/400977" @default.
• W82168767 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2670556" @default.
• W82168767 hasPublicationYear "1989" @default.
• W82168767 type Work @default.
• W82168767 sameAs 82168767 @default.
• W82168767 citedByCount "168" @default.
• W82168767 countsByYear W821687672012 @default.
• W82168767 countsByYear W821687672013 @default.
• W82168767 countsByYear W821687672014 @default.
• W82168767 countsByYear W821687672015 @default.
• W82168767 crossrefType "journal-article" @default.
• W82168767 hasAuthorship W82168767A5041664877 @default.
• W82168767 hasAuthorship W82168767A5067382234 @default.
• W82168767 hasBestOaLocation W821687672 @default.
• W82168767 hasConcept C121332964 @default.
• W82168767 hasConcept C148898269 @default.
• W82168767 hasConcept C153911025 @default.
• W82168767 hasConcept C190062978 @default.
• W82168767 hasConcept C2780114586 @default.
• W82168767 hasConcept C54355233 @default.
• W82168767 hasConcept C59157529 @default.
• W82168767 hasConcept C62520636 @default.
• W82168767 hasConcept C86803240 @default.
• W82168767 hasConceptScore W82168767C121332964 @default.
• W82168767 hasConceptScore W82168767C148898269 @default.
• W82168767 hasConceptScore W82168767C153911025 @default.
• W82168767 hasConceptScore W82168767C190062978 @default.
• W82168767 hasConceptScore W82168767C2780114586 @default.
• W82168767 hasConceptScore W82168767C54355233 @default.
• W82168767 hasConceptScore W82168767C59157529 @default.
• W82168767 hasConceptScore W82168767C62520636 @default.
• W82168767 hasConceptScore W82168767C86803240 @default.
• W82168767 hasIssue "5" @default.
• W82168767 hasLocation W821687671 @default.
• W82168767 hasLocation W821687672 @default.
• W82168767 hasLocation W821687673 @default.
• W82168767 hasOpenAccess W82168767 @default.
• W82168767 hasPrimaryLocation W821687671 @default.
• W82168767 hasRelatedWork W149313674 @default.
• W82168767 hasRelatedWork W1502573407 @default.
• W82168767 hasRelatedWork W1981278811 @default.
• W82168767 hasRelatedWork W1988350824 @default.
• W82168767 hasRelatedWork W1994669491 @default.
• W82168767 hasRelatedWork W2033049456 @default.
• W82168767 hasRelatedWork W2089790589 @default.
• W82168767 hasRelatedWork W2347972865 @default.
• W82168767 hasRelatedWork W4321446926 @default.
• W82168767 hasRelatedWork W4323285551 @default.
• W82168767 hasVolume "8" @default.
• W82168767 isParatext "false" @default.

• next