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- W821885181 abstract "With the ever-growing concern of water deficit due to global climatic change, the drought and salinity stressresponse of plants is a major area of research. However, the effect of these stress on cup-quality of coffeeespecially, the accumulation of caffeine biosynthetic metabolites, has not been documented. This work studiesthe methylxanthines (7-methylxanthine, theobromine, caffeine and theophylline) contents in young leaves ofcoffee in response to PEG-6000 (1.5% and 15% w/v) induced drought and sodium chloride (20mM and 200mM)induced salinity stress. In general, both the stress reduced the caffeine content except for 20mM NaCl. 1.5% PEGreduced caffeine by 0.46 fold and 0.57 fold during first 24hr and 48hr of treatment, respectively; PEG at 15%caused a reduction by 0.36 fold only in the 48hr of treatment compared to untreated plants; and NaCl at 200mMcaused a reduction of 0.26 fold and 0.47 fold in the first 24 and 48hrs of treatment, respectively. However 20mMNaCl augmented caffeine by 1.93 and 5.1 fold in the first 24 and 48hrs of treatment, respectively. The levels ofcaffeine subdued on the withdrawal of the stressor, affirmatively indicating the stress stimuli to be responsible forthe observed changes in caffeine levels. The biochemical profile was supported by transcript expression of thecaffeine biosynthetic NMT genes and the analysis of regulatory motifs of the promoters. The contents ofupstream methylxanthines (7-methylxanthine and theobromine) and the degradation pathway (theophylline)indicate that salinity and drought might have a negative impact on biosynthesis of caffeine but accelerated therate of caffeine degradation." @default.
- W821885181 created "2016-06-24" @default.
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- W821885181 date "2015-01-01" @default.
- W821885181 modified "2023-10-05" @default.
- W821885181 title "Salinity and drought response alleviate caffeine content of young leaves of Coffea canephora var. Robusta cv. S274" @default.
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- W821885181 doi "https://doi.org/10.7324/jabb.2015.3310" @default.
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