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- W822088993 abstract "Abstract The lipopolysaccharide (LPS) receptor complex of TLR4, MD-2 and CD14 plays an important role in sensing LPS-mediated immune signals, which provide immediate host protection and stimulate the host immune responses. Despite the fact that TLR4-mediated innate immune signals are required to combat invading pathogens, when signaling is not well regulated, uncontrolled activation can result in the disruption of host homeostasis leading to chronic inflammation and septic shock. Hence it is crucial to develop an assay system that can identify immunomodulators to control TLR4 signaling pathway. Since IL-8 is a major cytokine induced by TLR4, we developed the reporter cell line which stably expresses TLR4, MD-2 and CD14 proteins as well as an optimized Renilla luciferase reporter gene under the transcriptional control of IL-8 promoter. Functional activity of the cell line was evaluated by LPS dose response, TLR4 antagonist test and IL-8 induction suppressor test. The LPS EC50 of 4.3 ng/ml, TLR4 inhibitory peptide IC50 of 14.85 uM and an IL-8 induction suppressor, curcumin IC50 of 15.29 uM against the cell line were assessed. Using this cell line, a series of natural compounds was successfully screened for TLR4 signaling modulators, among which several compounds were identified as potential TLR4 signaling modulators. Taken together, it is proposed that this reporter cell line can be used as a tool for analyzing TLR4 signaling pathway as well as screening of TLR4 signaling modulators." @default.
- W822088993 created "2016-06-24" @default.
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- W822088993 date "2013-05-01" @default.
- W822088993 modified "2023-09-30" @default.
- W822088993 title "TLR4-based IL-8 reporter cell line as a tool for screening of the LPS-mediated immunomodulators to control TLR4 signaling pathway. (P3305)" @default.
- W822088993 doi "https://doi.org/10.4049/jimmunol.190.supp.211.10" @default.
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