Matches in SemOpenAlex for { <https://semopenalex.org/work/W823643731> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W823643731 abstract "This thesis is concerned primarily with peptide and protein partitioning in aqueous two-phase systems and the connection between partitioning behavior and protein structure. The surface properties of a protein is often crucial for recognition and interaction with other molecules. Important functional residues can be identified by mutational analysis. There is a need for rapid methods to study protein surfaces and surface changes due to mutations. Partitioning in aqueous two-phase systems has the potential to be used in this respect, since protein partitioning depends on the surface properties of the protein. However, we need to know how different amino acid residues contribute to the partition coefficient of a protein. Two aqueous phases can be formed by two structurally different polymers in aqueous solution. The polymers mainly used in this work have been thermoseparating ethylene oxide-propylene oxide random copolymers and dextran. The results in this thesis have shown the influence both qualitatively and quantitatively of different amino acid residues on the partitioning of proteins. Additionally, peptide partitioning could be used to predict the contribution of surface exposed amino acid residues to the partition coefficient of a protein. The effect on partitioning could be described only taking into account solvent accessibility and type of residue. Thus, aqueous two-phase partitioning can be used as a rapid method for studying the surface exposure of charged, hydrophobic, and especially aromatic amino acid residues. The knowledge gained can also be used to design efficient peptide tags for a target protein to direct it to a desired polymer phase. This technique can be used for large-scale purification of a protein. (Less)" @default.
- W823643731 created "2016-06-24" @default.
- W823643731 creator A5024673490 @default.
- W823643731 date "2000-01-01" @default.
- W823643731 modified "2023-09-25" @default.
- W823643731 title "Protein Surfaces Probed by Aqueous Two-phase Partitioning" @default.
- W823643731 hasPublicationYear "2000" @default.
- W823643731 type Work @default.
- W823643731 sameAs 823643731 @default.
- W823643731 citedByCount "0" @default.
- W823643731 crossrefType "dissertation" @default.
- W823643731 hasAuthorship W823643731A5024673490 @default.
- W823643731 hasConcept C11072593 @default.
- W823643731 hasConcept C114614502 @default.
- W823643731 hasConcept C147597530 @default.
- W823643731 hasConcept C149556010 @default.
- W823643731 hasConcept C178790620 @default.
- W823643731 hasConcept C184651966 @default.
- W823643731 hasConcept C185592680 @default.
- W823643731 hasConcept C192552737 @default.
- W823643731 hasConcept C2779281246 @default.
- W823643731 hasConcept C33923547 @default.
- W823643731 hasConcept C42812 @default.
- W823643731 hasConcept C515207424 @default.
- W823643731 hasConcept C521977710 @default.
- W823643731 hasConcept C55493867 @default.
- W823643731 hasConceptScore W823643731C11072593 @default.
- W823643731 hasConceptScore W823643731C114614502 @default.
- W823643731 hasConceptScore W823643731C147597530 @default.
- W823643731 hasConceptScore W823643731C149556010 @default.
- W823643731 hasConceptScore W823643731C178790620 @default.
- W823643731 hasConceptScore W823643731C184651966 @default.
- W823643731 hasConceptScore W823643731C185592680 @default.
- W823643731 hasConceptScore W823643731C192552737 @default.
- W823643731 hasConceptScore W823643731C2779281246 @default.
- W823643731 hasConceptScore W823643731C33923547 @default.
- W823643731 hasConceptScore W823643731C42812 @default.
- W823643731 hasConceptScore W823643731C515207424 @default.
- W823643731 hasConceptScore W823643731C521977710 @default.
- W823643731 hasConceptScore W823643731C55493867 @default.
- W823643731 hasLocation W8236437311 @default.
- W823643731 hasOpenAccess W823643731 @default.
- W823643731 hasPrimaryLocation W8236437311 @default.
- W823643731 hasRelatedWork W10059953 @default.
- W823643731 hasRelatedWork W1015598414 @default.
- W823643731 hasRelatedWork W112391118 @default.
- W823643731 hasRelatedWork W120492510 @default.
- W823643731 hasRelatedWork W1696690880 @default.
- W823643731 hasRelatedWork W1967428393 @default.
- W823643731 hasRelatedWork W1980336289 @default.
- W823643731 hasRelatedWork W1991251540 @default.
- W823643731 hasRelatedWork W2012024491 @default.
- W823643731 hasRelatedWork W2032779282 @default.
- W823643731 hasRelatedWork W2077108500 @default.
- W823643731 hasRelatedWork W2169178078 @default.
- W823643731 hasRelatedWork W2415329377 @default.
- W823643731 hasRelatedWork W2467872366 @default.
- W823643731 hasRelatedWork W2472328292 @default.
- W823643731 hasRelatedWork W2805290131 @default.
- W823643731 hasRelatedWork W3210314250 @default.
- W823643731 hasRelatedWork W1511098098 @default.
- W823643731 hasRelatedWork W1541353694 @default.
- W823643731 hasRelatedWork W2969015857 @default.
- W823643731 isParatext "false" @default.
- W823643731 isRetracted "false" @default.
- W823643731 magId "823643731" @default.
- W823643731 workType "dissertation" @default.