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- W825506959 abstract "Enzyme-linked immunosorbent assay was developed for the analysis of soy protein in foods. Competitive indirect ELISA (ciELISA) was established by using specific antibodies against the heat-stable acidic subunits (AS) of glycinin. Soy proteins in each sample used in this study were solublized in the presence of urea and DTT and boiled at for 1hr and then were renatured with a cystine-containing solution. After these treatments, each isolated soy protein (ISP) heated at 60, 70, 80, for 10 minutes showed almost the same curve as unheated one in the ciELISA. The detection limit of ISP was 0.3 . Anti-AS antibodies have very low reactivities less than 0.1% toward non-meat proteins such as skim milk and casein and did not show any reactivities toward egg white powder and ovalbumin. When laboratory-made sausages containing ISP of were assayed by ciELISA, the mean recovery was about 83% (C.V., 19%). In addition, the average content of soy protein in commercial sausages was 1.27%." @default.
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- W825506959 date "2000-01-01" @default.
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- W825506959 title "An Enzyme-Linked Immunosorbent Assay for Quantitation of Soy Proteins in Food" @default.
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