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- W82564384 abstract "Objective: To identify the temporal gene expression of asporin, one of the extracellular matrix (ECM) molecules, during mouse alveogenesis or lung alveolar formation. Methods: C57BL/6 mouse lungs at postnatal days (P) 1, 7, 14, 21, 28 and 60 were harvested. RNA purification and quantita- tive real time - PCR were used for indentifying the temporal pattern of asporin gene expression. Mouse lungs were inflated with 10% buffered formalin at 25 cmH2 O and further processed for histological study of airspace development. Results: The peak of asporin gene expression in mouse lungs was observed on P14, which related to the alveolar stage of mouse lung development. The level of asporin gene expression on P14 was significantly elevated, twentyfold and sixtyfold higher, in comparison with P1 (saccular stage) and P60 (adult age), respectively. Alveogenesis was also observed from mouse lung histology on P7 and P14. Conclusion: Highly regulated asporin expression in the lung during alveogenesis suggested that asporin expression affects alveogenesis" @default.
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- W82564384 date "2017-04-04" @default.
- W82564384 modified "2023-09-24" @default.
- W82564384 title "Asporin Gene Expression in Mouse Alveogenesis" @default.
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