Matches in SemOpenAlex for { <https://semopenalex.org/work/W8271612> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W8271612 endingPage "92" @default.
- W8271612 startingPage "289" @default.
- W8271612 abstract "To rapidly detect SOX2 gene using primed in situ labeling (PRINS).Human peripheral blood samples were cultured using an optimized method. Sequence of the SOX2 gene was amplified in situ with biotin-labeled specific primers and processed with a tyramide signal amplification (TSA) biotin system. Subsequently, fluorescence-stained signal was detected by streptavidin-Texas red. For the control group, MCF-10F cells were transfected with Lentivirus hSox2.By VideoTesT-FISH software analysis, the long arm of chromosome 3 in the experimental group showed a specific red fluorescence signal, whilst the control samples showed no specific signals for SOX2. Transfected MCF-10F cells showed various efficiency of SOX2 gene integration.PRINS utilizes a highly sensitive in situ PCR technique combined with fluorescence labeled oligodeoxynucleotides can synthesize probes in situ, thus greatly reducing the cost of probe and time for detection. It can facilitate identification and classification of induced pluripotent stem cells, and has many potential applications in this prospect." @default.
- W8271612 created "2016-06-24" @default.
- W8271612 creator A5018696698 @default.
- W8271612 creator A5025278311 @default.
- W8271612 creator A5047664457 @default.
- W8271612 creator A5088356815 @default.
- W8271612 date "2012-06-01" @default.
- W8271612 modified "2023-10-18" @default.
- W8271612 title "[Rapid detection of SOX2 gene by primed in situ labeling]." @default.
- W8271612 doi "https://doi.org/10.3760/cma.j.issn.1003-9406.2012.03.009" @default.
- W8271612 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22678791" @default.
- W8271612 hasPublicationYear "2012" @default.
- W8271612 type Work @default.
- W8271612 sameAs 8271612 @default.
- W8271612 citedByCount "0" @default.
- W8271612 crossrefType "journal-article" @default.
- W8271612 hasAuthorship W8271612A5018696698 @default.
- W8271612 hasAuthorship W8271612A5025278311 @default.
- W8271612 hasAuthorship W8271612A5047664457 @default.
- W8271612 hasAuthorship W8271612A5088356815 @default.
- W8271612 hasConcept C104317684 @default.
- W8271612 hasConcept C121332964 @default.
- W8271612 hasConcept C145103041 @default.
- W8271612 hasConcept C150194340 @default.
- W8271612 hasConcept C153911025 @default.
- W8271612 hasConcept C178790620 @default.
- W8271612 hasConcept C185592680 @default.
- W8271612 hasConcept C204628709 @default.
- W8271612 hasConcept C2776190903 @default.
- W8271612 hasConcept C2777542201 @default.
- W8271612 hasConcept C2777822432 @default.
- W8271612 hasConcept C2777933648 @default.
- W8271612 hasConcept C2778204606 @default.
- W8271612 hasConcept C30481170 @default.
- W8271612 hasConcept C54009773 @default.
- W8271612 hasConcept C55493867 @default.
- W8271612 hasConcept C62520636 @default.
- W8271612 hasConcept C81439078 @default.
- W8271612 hasConcept C86803240 @default.
- W8271612 hasConcept C91881484 @default.
- W8271612 hasConceptScore W8271612C104317684 @default.
- W8271612 hasConceptScore W8271612C121332964 @default.
- W8271612 hasConceptScore W8271612C145103041 @default.
- W8271612 hasConceptScore W8271612C150194340 @default.
- W8271612 hasConceptScore W8271612C153911025 @default.
- W8271612 hasConceptScore W8271612C178790620 @default.
- W8271612 hasConceptScore W8271612C185592680 @default.
- W8271612 hasConceptScore W8271612C204628709 @default.
- W8271612 hasConceptScore W8271612C2776190903 @default.
- W8271612 hasConceptScore W8271612C2777542201 @default.
- W8271612 hasConceptScore W8271612C2777822432 @default.
- W8271612 hasConceptScore W8271612C2777933648 @default.
- W8271612 hasConceptScore W8271612C2778204606 @default.
- W8271612 hasConceptScore W8271612C30481170 @default.
- W8271612 hasConceptScore W8271612C54009773 @default.
- W8271612 hasConceptScore W8271612C55493867 @default.
- W8271612 hasConceptScore W8271612C62520636 @default.
- W8271612 hasConceptScore W8271612C81439078 @default.
- W8271612 hasConceptScore W8271612C86803240 @default.
- W8271612 hasConceptScore W8271612C91881484 @default.
- W8271612 hasIssue "3" @default.
- W8271612 hasLocation W82716121 @default.
- W8271612 hasOpenAccess W8271612 @default.
- W8271612 hasPrimaryLocation W82716121 @default.
- W8271612 hasRelatedWork W1965639115 @default.
- W8271612 hasRelatedWork W1972824801 @default.
- W8271612 hasRelatedWork W1992657368 @default.
- W8271612 hasRelatedWork W2086514592 @default.
- W8271612 hasRelatedWork W2092900161 @default.
- W8271612 hasRelatedWork W2094296784 @default.
- W8271612 hasRelatedWork W2155239658 @default.
- W8271612 hasRelatedWork W2329741613 @default.
- W8271612 hasRelatedWork W2407145548 @default.
- W8271612 hasRelatedWork W2462922943 @default.
- W8271612 hasVolume "29" @default.
- W8271612 isParatext "false" @default.
- W8271612 isRetracted "false" @default.
- W8271612 magId "8271612" @default.
- W8271612 workType "article" @default.