Matches in SemOpenAlex for { <https://semopenalex.org/work/W827676559> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W827676559 abstract "Single-molecule force spectroscopy provides insights into the energetics and unfolding of individual proteins and nucleic acid structures. Using the techniques described below, we can now study the mechanical unfolding and re-folding of bacteriorhodopsin (BR), a model membrane protein, in its native lipid bi-layer. In a typical force spectroscopy experiment, an AFM tip is coupled to a surface-adsorbed protein by pressing the tip into it. Force-extension curves are then generated by retracting the tip at a constant velocity using a piezoelectric (PZT) stage. Force is measured by cantilever deflection. Extension, or more precisely tip-sample separation, is deduced from the PZT stage position used to control the vertical tip position. Thus, this deduced extension is sensitive to the vertical mechanical drift of the AFM assembly (~10 nm/min). We developed an ultrastable AFM in which the tip and the sample positions are independently measured by, and stabilized with respect to, a pair of laser foci in three dimensions. These lasers establish a local reference frame that is insensitive to long-term mechanical drift of the AFM assembly. The resulting AFM was 100-fold more stable than prior state of the art and tip position was controlled to 0.3Å in 3D. We have extended the ultrastable AFM into liquid and can mechanically unfold proteins at slow, but actively, stabilized pulling velocities (2.5 Å/s). We can also stop pulling altogether and hold the molecule at constant force while independently measuring tip-sample separation. I will illustrate the instrument's potential with preliminary force spectroscopy results on bacteriorhodopsin in which we observed the folding and unfolding of a membrane protein into and out of its native membrane over hundreds of seconds. Finally, I will discuss technical improvements to our ultra-stable AFM to facilitate finding sparsely distributed unlabeled biological samples." @default.
- W827676559 created "2016-06-24" @default.
- W827676559 creator A5007181594 @default.
- W827676559 date "2012-01-01" @default.
- W827676559 modified "2023-09-27" @default.
- W827676559 title "An Ultrastable AFM for Biophysics" @default.
- W827676559 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3630666" @default.
- W827676559 hasPublicationYear "2012" @default.
- W827676559 type Work @default.
- W827676559 sameAs 827676559 @default.
- W827676559 citedByCount "0" @default.
- W827676559 crossrefType "journal-article" @default.
- W827676559 hasAuthorship W827676559A5007181594 @default.
- W827676559 hasConcept C100082104 @default.
- W827676559 hasConcept C102951782 @default.
- W827676559 hasConcept C113196181 @default.
- W827676559 hasConcept C119473381 @default.
- W827676559 hasConcept C141354745 @default.
- W827676559 hasConcept C159467904 @default.
- W827676559 hasConcept C159985019 @default.
- W827676559 hasConcept C171250308 @default.
- W827676559 hasConcept C185592680 @default.
- W827676559 hasConcept C189542057 @default.
- W827676559 hasConcept C192562407 @default.
- W827676559 hasConcept C41625074 @default.
- W827676559 hasConcept C43617362 @default.
- W827676559 hasConcept C55493867 @default.
- W827676559 hasConceptScore W827676559C100082104 @default.
- W827676559 hasConceptScore W827676559C102951782 @default.
- W827676559 hasConceptScore W827676559C113196181 @default.
- W827676559 hasConceptScore W827676559C119473381 @default.
- W827676559 hasConceptScore W827676559C141354745 @default.
- W827676559 hasConceptScore W827676559C159467904 @default.
- W827676559 hasConceptScore W827676559C159985019 @default.
- W827676559 hasConceptScore W827676559C171250308 @default.
- W827676559 hasConceptScore W827676559C185592680 @default.
- W827676559 hasConceptScore W827676559C189542057 @default.
- W827676559 hasConceptScore W827676559C192562407 @default.
- W827676559 hasConceptScore W827676559C41625074 @default.
- W827676559 hasConceptScore W827676559C43617362 @default.
- W827676559 hasConceptScore W827676559C55493867 @default.
- W827676559 hasLocation W8276765591 @default.
- W827676559 hasOpenAccess W827676559 @default.
- W827676559 hasPrimaryLocation W8276765591 @default.
- W827676559 hasRelatedWork W118412929 @default.
- W827676559 hasRelatedWork W1491361148 @default.
- W827676559 hasRelatedWork W1558977365 @default.
- W827676559 hasRelatedWork W1766475007 @default.
- W827676559 hasRelatedWork W1973612517 @default.
- W827676559 hasRelatedWork W1975655661 @default.
- W827676559 hasRelatedWork W1994430704 @default.
- W827676559 hasRelatedWork W1997775709 @default.
- W827676559 hasRelatedWork W2016446191 @default.
- W827676559 hasRelatedWork W2159160861 @default.
- W827676559 hasRelatedWork W2186805170 @default.
- W827676559 hasRelatedWork W2260625346 @default.
- W827676559 hasRelatedWork W2760229912 @default.
- W827676559 hasRelatedWork W2794002510 @default.
- W827676559 hasRelatedWork W2795962061 @default.
- W827676559 hasRelatedWork W32403697 @default.
- W827676559 hasRelatedWork W61467147 @default.
- W827676559 hasRelatedWork W752081210 @default.
- W827676559 hasRelatedWork W92074886 @default.
- W827676559 hasRelatedWork W2608565914 @default.
- W827676559 hasVolume "23" @default.
- W827676559 isParatext "false" @default.
- W827676559 isRetracted "false" @default.
- W827676559 magId "827676559" @default.
- W827676559 workType "article" @default.