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- W82818152 abstract "Objective To find out a molecular marker which could differentiate the diet-induced-obesity(DIO) rats and control rats,and probe into the relationship between microbiota structure and obesity.Method ERIC-PCR combined with Southern-blot were applied to obtain fingerprint profiles,and then the band that was unique to the DIO group was sequenced.Specific primers were designed based on the sequenced DNA fragment,and used for quantitative PCR to verify the distribution of this DNA fragment in microflora of the two animal groups.Result This experiment indicated the difference of microbiota structure between the DIO and control rats.Besides,a genomic DNA fragment was detected only in DIO group,and the discrepancy between the two groups was found by quantitative PCR to be significant.Conclusion A genomic DNA fragment,which was unique to DIO group and was a key differentiator to distinguish normal rats,could be used as a molecular marker to study the mechanism of diet-induced-obesity." @default.
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- W82818152 date "2009-01-01" @default.
- W82818152 modified "2023-09-23" @default.
- W82818152 title "Intestinal microbiota structure of diet-induced obesity rats and its specific molecular marker." @default.
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