Matches in SemOpenAlex for { <https://semopenalex.org/work/W828231454> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W828231454 abstract "Glycoproteins, a class of biomolecules, exhibit a variety of biological activities and therefore are of importance in modern biotechnology and pharmaceutical industry. Pure glycoproteins are needed for structural and functional studies. However, the separation of glycoproteins has been a problem until now due to their structural complexity. Efficient separation techniques are thus required.This thesis presents a novel technique, shielding boronate affinity chromatography (SBAC), which eliminates non-specific boronate–protein interactions via introducing a so-called shielding reagent into the mobile phase. To be a suitable shielding reagent, the following requirements need to be fulfilled: (1) the shielding reagent should have an affinity to the boronate ligand and should not interact with the sample molecules to be purified; (2) the boronate–shielding reagent interactions must be stronger than non-specific boronate–protein interactions, while weaker than specific boronate-glycoprotein interactions; and (3) the shielding reagent should be non-toxic and chemi-cally stable in order to be applied to pharmaceutical industry. The shielding efficiency of a shiel-ding reagent is correlated to its chemical structure. Low-molecular-mass polyhydroxyl compounds with a conformation that allows the formation of tridentate complexes with the boronate anion exhibit the highest shielding efficiencies. Such compounds can also function as eluting agents when used at high concentrations.The feasibility of using SBAC to the separation of glycoproteins is demonstrated through several examples, such as (1) separation of a neoglycoprotein, maltose-modified Cht (Cht-Mal), from non-glycosylated Cht; (2) separation of Cht-Mal into individual fractions with different degrees of glycosylation; (3) separation of two mistletoe lectins from each other based on their degree of glycosylation; and (4) separation of glycated haemoglobin (GHb) from non-glycated Hb species as well as separation of GHb into two fractions that exhibit different glycation patterns. The study shows that SBAC has higher separation resolution than conventional boronate affinity chromatography." @default.
- W828231454 created "2016-06-24" @default.
- W828231454 creator A5029953609 @default.
- W828231454 date "2001-01-01" @default.
- W828231454 modified "2023-09-27" @default.
- W828231454 title "Separation of Glycoproteins using Shielding Boronate Affinity Chromatography" @default.
- W828231454 hasPublicationYear "2001" @default.
- W828231454 type Work @default.
- W828231454 sameAs 828231454 @default.
- W828231454 citedByCount "0" @default.
- W828231454 crossrefType "dissertation" @default.
- W828231454 hasAuthorship W828231454A5029953609 @default.
- W828231454 hasConcept C108625454 @default.
- W828231454 hasConcept C113078167 @default.
- W828231454 hasConcept C116569031 @default.
- W828231454 hasConcept C159985019 @default.
- W828231454 hasConcept C170493617 @default.
- W828231454 hasConcept C178790620 @default.
- W828231454 hasConcept C181199279 @default.
- W828231454 hasConcept C185592680 @default.
- W828231454 hasConcept C192562407 @default.
- W828231454 hasConcept C21951064 @default.
- W828231454 hasConcept C2265751 @default.
- W828231454 hasConcept C2779227376 @default.
- W828231454 hasConcept C40875361 @default.
- W828231454 hasConcept C43617362 @default.
- W828231454 hasConcept C49853544 @default.
- W828231454 hasConcept C55493867 @default.
- W828231454 hasConcept C58121356 @default.
- W828231454 hasConceptScore W828231454C108625454 @default.
- W828231454 hasConceptScore W828231454C113078167 @default.
- W828231454 hasConceptScore W828231454C116569031 @default.
- W828231454 hasConceptScore W828231454C159985019 @default.
- W828231454 hasConceptScore W828231454C170493617 @default.
- W828231454 hasConceptScore W828231454C178790620 @default.
- W828231454 hasConceptScore W828231454C181199279 @default.
- W828231454 hasConceptScore W828231454C185592680 @default.
- W828231454 hasConceptScore W828231454C192562407 @default.
- W828231454 hasConceptScore W828231454C21951064 @default.
- W828231454 hasConceptScore W828231454C2265751 @default.
- W828231454 hasConceptScore W828231454C2779227376 @default.
- W828231454 hasConceptScore W828231454C40875361 @default.
- W828231454 hasConceptScore W828231454C43617362 @default.
- W828231454 hasConceptScore W828231454C49853544 @default.
- W828231454 hasConceptScore W828231454C55493867 @default.
- W828231454 hasConceptScore W828231454C58121356 @default.
- W828231454 hasLocation W8282314541 @default.
- W828231454 hasOpenAccess W828231454 @default.
- W828231454 hasPrimaryLocation W8282314541 @default.
- W828231454 hasRelatedWork W1416261912 @default.
- W828231454 hasRelatedWork W1437483034 @default.
- W828231454 hasRelatedWork W1529926784 @default.
- W828231454 hasRelatedWork W156125412 @default.
- W828231454 hasRelatedWork W1587073416 @default.
- W828231454 hasRelatedWork W1903005968 @default.
- W828231454 hasRelatedWork W1989278383 @default.
- W828231454 hasRelatedWork W2028733979 @default.
- W828231454 hasRelatedWork W2049451264 @default.
- W828231454 hasRelatedWork W2084459739 @default.
- W828231454 hasRelatedWork W2088693813 @default.
- W828231454 hasRelatedWork W2094112806 @default.
- W828231454 hasRelatedWork W2282824963 @default.
- W828231454 hasRelatedWork W237270168 @default.
- W828231454 hasRelatedWork W2964128692 @default.
- W828231454 hasRelatedWork W2990802048 @default.
- W828231454 hasRelatedWork W3026069318 @default.
- W828231454 hasRelatedWork W3098619839 @default.
- W828231454 hasRelatedWork W3155785609 @default.
- W828231454 hasRelatedWork W3204024892 @default.
- W828231454 isParatext "false" @default.
- W828231454 isRetracted "false" @default.
- W828231454 magId "828231454" @default.
- W828231454 workType "dissertation" @default.