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- W828563802 abstract "A fluorescence tagging agent, FMOC-Cl (9-fluorenylmethyl chloroformate) was used for the determination of 1-amino-oligosaccharide intermediates generated from glycoproteins by peptide-N asparagine amidase (N-Glycanase, PNGase F). The derivatives were separated on an Amido 80 column by HPLC using a gradient system with 25 to 51% aqueous acetonitrile and monitored by a fluorometric detector. The detection limit of FMOC-amino-oligosaccharides was pmol with fluorometric detection at 278 nm." @default.
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- W828563802 date "1997-01-01" @default.
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- W828563802 title "Application of FMOC-Cl for the Quantitative Determination of N-linked Oligosaccharides" @default.
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