Matches in SemOpenAlex for { <https://semopenalex.org/work/W82869603> ?p ?o ?g. }
- W82869603 endingPage "7668" @default.
- W82869603 startingPage "7663" @default.
- W82869603 abstract "Abstract Endothelial cells from brain microvessels form the blood-brain barrier. Brain microvessels and endothelial cells isolated from rat brain microvessels express an amiloride-sensitive cationic channel that was characterized using [3H]phenamil binding and patch-clamp experiments. [3H]Phenamil, a labeled amiloride analog, recognizes a single family of binding sites with a dissociation constant of 20-30 nM and a maximum binding capacity of 8-15 pmol/mg protein. The pharmacological profile of the channel (phenamil greater than benzamil greater than amiloride) is very similar to that of the epithelium Na+ channel of mammalian kidney and of frog epithelia. Long-lasting currents were observed in patch-clamp experiments using excised outside-out patches. Application of amiloride or phenamil first produced a rapid flickering of channel activity and then its complete blockade. The mean unit channel conductance at 140 mM Na+ was 23 picosiemens. The selectivity of Na+ over K+ was estimated from reversal potentials to be 1.5:1. Properties of the channel in microvessels are clearly distinct from those of the Na+ channel of the kidney, suggesting the existence of several isoforms of cationic channels that are sensitive to amiloride and its derivatives. The low selectivity cationic channel of endothelial cells in brain microvessels might be important for controlling both Na+ and K+ movements across the blood-brain barrier." @default.
- W82869603 created "2016-06-24" @default.
- W82869603 creator A5041868402 @default.
- W82869603 creator A5044327360 @default.
- W82869603 creator A5062264648 @default.
- W82869603 creator A5068045779 @default.
- W82869603 creator A5078621084 @default.
- W82869603 creator A5084982109 @default.
- W82869603 date "1989-05-01" @default.
- W82869603 modified "2023-10-15" @default.
- W82869603 title "A new type of amiloride-sensitive cationic channel in endothelial cells of brain microvessels" @default.
- W82869603 cites W1533306569 @default.
- W82869603 cites W1674029742 @default.
- W82869603 cites W1977940746 @default.
- W82869603 cites W1992407119 @default.
- W82869603 cites W1996551334 @default.
- W82869603 cites W1998680286 @default.
- W82869603 cites W2016147051 @default.
- W82869603 cites W2024214678 @default.
- W82869603 cites W2024400996 @default.
- W82869603 cites W2026037618 @default.
- W82869603 cites W2048306533 @default.
- W82869603 cites W2050285850 @default.
- W82869603 cites W2053746701 @default.
- W82869603 cites W2059437136 @default.
- W82869603 cites W2061096622 @default.
- W82869603 cites W2063441247 @default.
- W82869603 cites W2074548865 @default.
- W82869603 cites W2076634690 @default.
- W82869603 cites W2078285509 @default.
- W82869603 cites W2084725128 @default.
- W82869603 cites W2103252057 @default.
- W82869603 cites W2108089336 @default.
- W82869603 cites W2146109103 @default.
- W82869603 cites W2152353759 @default.
- W82869603 cites W2318996479 @default.
- W82869603 cites W2338111940 @default.
- W82869603 cites W2409020021 @default.
- W82869603 cites W2415927659 @default.
- W82869603 cites W2914333680 @default.
- W82869603 cites W4249822231 @default.
- W82869603 cites W4293247451 @default.
- W82869603 cites W7713465 @default.
- W82869603 doi "https://doi.org/10.1016/s0021-9258(18)83285-9" @default.
- W82869603 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2468671" @default.
- W82869603 hasPublicationYear "1989" @default.
- W82869603 type Work @default.
- W82869603 sameAs 82869603 @default.
- W82869603 citedByCount "129" @default.
- W82869603 countsByYear W828696032012 @default.
- W82869603 countsByYear W828696032013 @default.
- W82869603 countsByYear W828696032014 @default.
- W82869603 countsByYear W828696032015 @default.
- W82869603 countsByYear W828696032021 @default.
- W82869603 crossrefType "journal-article" @default.
- W82869603 hasAuthorship W82869603A5041868402 @default.
- W82869603 hasAuthorship W82869603A5044327360 @default.
- W82869603 hasAuthorship W82869603A5062264648 @default.
- W82869603 hasAuthorship W82869603A5068045779 @default.
- W82869603 hasAuthorship W82869603A5078621084 @default.
- W82869603 hasAuthorship W82869603A5084982109 @default.
- W82869603 hasBestOaLocation W828696031 @default.
- W82869603 hasConcept C12554922 @default.
- W82869603 hasConcept C127162648 @default.
- W82869603 hasConcept C169760540 @default.
- W82869603 hasConcept C178790620 @default.
- W82869603 hasConcept C183882617 @default.
- W82869603 hasConcept C185592680 @default.
- W82869603 hasConcept C2779608910 @default.
- W82869603 hasConcept C31258907 @default.
- W82869603 hasConcept C41008148 @default.
- W82869603 hasConcept C537181965 @default.
- W82869603 hasConcept C6507245 @default.
- W82869603 hasConcept C86803240 @default.
- W82869603 hasConcept C95444343 @default.
- W82869603 hasConceptScore W82869603C12554922 @default.
- W82869603 hasConceptScore W82869603C127162648 @default.
- W82869603 hasConceptScore W82869603C169760540 @default.
- W82869603 hasConceptScore W82869603C178790620 @default.
- W82869603 hasConceptScore W82869603C183882617 @default.
- W82869603 hasConceptScore W82869603C185592680 @default.
- W82869603 hasConceptScore W82869603C2779608910 @default.
- W82869603 hasConceptScore W82869603C31258907 @default.
- W82869603 hasConceptScore W82869603C41008148 @default.
- W82869603 hasConceptScore W82869603C537181965 @default.
- W82869603 hasConceptScore W82869603C6507245 @default.
- W82869603 hasConceptScore W82869603C86803240 @default.
- W82869603 hasConceptScore W82869603C95444343 @default.
- W82869603 hasIssue "13" @default.
- W82869603 hasLocation W828696031 @default.
- W82869603 hasOpenAccess W82869603 @default.
- W82869603 hasPrimaryLocation W828696031 @default.
- W82869603 hasRelatedWork W133291183 @default.
- W82869603 hasRelatedWork W1629156103 @default.
- W82869603 hasRelatedWork W1976830450 @default.
- W82869603 hasRelatedWork W2004705636 @default.
- W82869603 hasRelatedWork W2015005230 @default.
- W82869603 hasRelatedWork W2079127109 @default.