Matches in SemOpenAlex for { <https://semopenalex.org/work/W829478918> ?p ?o ?g. }
- W829478918 endingPage "1193" @default.
- W829478918 startingPage "1177" @default.
- W829478918 abstract "Pichia pastoris, a methylotrophic yeast, is an established system for the production of heterologous proteins, particularly biopharmaceuticals and industrial enzymes. To maximise and optimise the production of recombinant products, recent molecular research has focused on numerous issues including the design of expression vectors, optimisation of gene copy number, co-expression of secretory proteins such as chaperones, engineering of glycosylation and secretory pathways, etc. However, the physiological effects of different cultivation strategies are often difficult to separate from the molecular effects of the gene construct (e.g., cellular stress through over-expression or incorrect post-translational processing). Hence, overall system optimisation is difficult, even though it is urgently required in order to describe and understand the behaviour of new molecular constructs. This review focuses on particular aspects of recombinant protein production related to variations in biomass growth and their implications for strain design and screening, as well as on the concept of rational comparisons between cultivation systems for the development of specific production processes in bioreactors. The relationship between specific formation rates of secreted recombinant proteins, qp, and specific growth rates, μ, has been analysed in a conceptual attempt to compare different systems, particularly those based on AOX1/methanol and GAP/glucose, and this has now evolved into a pivotal concept for bioprocess engineering of P. pastoris." @default.
- W829478918 created "2016-06-24" @default.
- W829478918 creator A5021120268 @default.
- W829478918 creator A5030809764 @default.
- W829478918 creator A5035848357 @default.
- W829478918 creator A5049034238 @default.
- W829478918 creator A5052958687 @default.
- W829478918 creator A5066970339 @default.
- W829478918 creator A5077805211 @default.
- W829478918 creator A5083643225 @default.
- W829478918 date "2015-11-01" @default.
- W829478918 modified "2023-10-01" @default.
- W829478918 title "Cultivation strategies to enhance productivity of Pichia pastoris: A review" @default.
- W829478918 cites W131662692 @default.
- W829478918 cites W1491481482 @default.
- W829478918 cites W1497462806 @default.
- W829478918 cites W1516116810 @default.
- W829478918 cites W1571221450 @default.
- W829478918 cites W1595540384 @default.
- W829478918 cites W1869516088 @default.
- W829478918 cites W1873692790 @default.
- W829478918 cites W1880125236 @default.
- W829478918 cites W1964146327 @default.
- W829478918 cites W1967073269 @default.
- W829478918 cites W1967500040 @default.
- W829478918 cites W1967681711 @default.
- W829478918 cites W1970228759 @default.
- W829478918 cites W1971680669 @default.
- W829478918 cites W1975687798 @default.
- W829478918 cites W1975895758 @default.
- W829478918 cites W1976021580 @default.
- W829478918 cites W1976208842 @default.
- W829478918 cites W1976330864 @default.
- W829478918 cites W1976429947 @default.
- W829478918 cites W1985597601 @default.
- W829478918 cites W1987023614 @default.
- W829478918 cites W1991656269 @default.
- W829478918 cites W1992412963 @default.
- W829478918 cites W1994506288 @default.
- W829478918 cites W1994545731 @default.
- W829478918 cites W1996790351 @default.
- W829478918 cites W1997480931 @default.
- W829478918 cites W1998058026 @default.
- W829478918 cites W2001195805 @default.
- W829478918 cites W2001381108 @default.
- W829478918 cites W2002018907 @default.
- W829478918 cites W2003319961 @default.
- W829478918 cites W2005767394 @default.
- W829478918 cites W2006671970 @default.
- W829478918 cites W2010155325 @default.
- W829478918 cites W2010593999 @default.
- W829478918 cites W2010986041 @default.
- W829478918 cites W2011449819 @default.
- W829478918 cites W2011498028 @default.
- W829478918 cites W2013231418 @default.
- W829478918 cites W2013636334 @default.
- W829478918 cites W2013849396 @default.
- W829478918 cites W2019021421 @default.
- W829478918 cites W2020007728 @default.
- W829478918 cites W2020149962 @default.
- W829478918 cites W2028875171 @default.
- W829478918 cites W2029780957 @default.
- W829478918 cites W2030899178 @default.
- W829478918 cites W2032988631 @default.
- W829478918 cites W2035509141 @default.
- W829478918 cites W2041370823 @default.
- W829478918 cites W2043748679 @default.
- W829478918 cites W2044272062 @default.
- W829478918 cites W2046437093 @default.
- W829478918 cites W2046798764 @default.
- W829478918 cites W2056304926 @default.
- W829478918 cites W2060504841 @default.
- W829478918 cites W2064897009 @default.
- W829478918 cites W2067370452 @default.
- W829478918 cites W2069890541 @default.
- W829478918 cites W2071149114 @default.
- W829478918 cites W2071195921 @default.
- W829478918 cites W2071247456 @default.
- W829478918 cites W2071768512 @default.
- W829478918 cites W2073297072 @default.
- W829478918 cites W2075762672 @default.
- W829478918 cites W2076041897 @default.
- W829478918 cites W2078389865 @default.
- W829478918 cites W2080430544 @default.
- W829478918 cites W2081051355 @default.
- W829478918 cites W2082581989 @default.
- W829478918 cites W2083281099 @default.
- W829478918 cites W2084327369 @default.
- W829478918 cites W2085595424 @default.
- W829478918 cites W2088691956 @default.
- W829478918 cites W2093657906 @default.
- W829478918 cites W2104113704 @default.
- W829478918 cites W2104510025 @default.
- W829478918 cites W2106237345 @default.
- W829478918 cites W2106911235 @default.
- W829478918 cites W2107238882 @default.
- W829478918 cites W2108786034 @default.
- W829478918 cites W2109587594 @default.