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• W829931305 abstract "A b s t r a c t This paper presents the results of research preformed on the biodiversity of bacteria at different depths of lab-scale con structed wetlands (CWs) systems loaded with water simulating electroplating wastewater. The bacterial biocenosis in two constructed wetland systems with different organic or mineral bed media was investigated. Samples were taken from the two columns to the metered synthetic galvanic wastewater, which included metals such as lead, nickel, zinc, copper and lac tates, and cyanides. Samples were taken after 53 weeks of the experiment. The biodiversity analysis was performed on the basis of distribution of DNA fragments coding for bacterial 16S rRNA by denaturing gradient gel electrophoresis. Based on the bioinformatic analysis of the obtained fingerprints Shannon biodiversity index was calculated to estimate species diver sity, and Jaccard index was determined, which allowed comparison of two communities. The study indicated that the two communities were significantly different, with a higher diversity in the column with a peat mixture S t r e s z c z e n i e W artykule przedstawiono wyniki badan dotyczących bioroznorodności bakterii na roznych glebokościach wypelnienia zloz hydrofitowych wykorzystywanych w celu oczyszczania ściekow. Badano biocenoze bakteryjną dwoch zloz o wypelnieniu organicznym oraz mineralnym. Probki pobrano z dwoch kolumn, do ktorych dozowano syntetyczne ścieki galwanizerskie, w sklad ktorych wchodzily metale, takie jak: olow, nikiel, cynk, miedź oraz mleczany, a takze cyjanki. Probki pobrano po 53 tygodniach pracy zloza.Analize bioroznorodności wykonano na podstawie wynikow rozdzialu fragmentow DNA kodują cych bakteryjne 16S rRNA za pomocą elektroforezy w gradiencie czynnika denaturującego. Na podstawie analizy bioinfor matycznej uzyskanego wzoru prązkowego (tzw. fingerprintu) wyznaczono wspolczynnik bioroznorodności Shannona, stosowany do szacowania roznorodności gatunkowej, a takze Indeks Jaccarda umozliwiający porownanie dwoch zbiorowisk bakterii. Uzyskane wyniki wykazaly, ze w kolumnie z wypelnieniem organicznym (torfem) bioroznorodnośc bakterii jest wieksza w porownaniu z kolumną z wypelnienieem mineralnym." @default.
• W829931305 created "2016-06-24" @default.
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• W829931305 date "2014-01-01" @default.
• W829931305 modified "2023-09-26" @default.
• W829931305 title "Depth distribution of bacterial diversity in lab scale up flow constructed wetlands" @default.
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