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- W83035099 abstract "Objective To investigate the protective effect and molecular mechanism of minocycline on toxicity of retinal ganglion cells induced by L-glutamate. Methods Primary mouse retinal ganglion cells (RGC) were isolated from mouse retinalin vitro. RGC were divided into control group, L-glutamate group,and L-glutamate + minocycline group, the cell survival rate and nerve axon growth length were observed. In vivo study, B6 mice was intravitreal injected with 2 μl L-glutamate (2 mmol/L) to construct a toxic damage to retinal ganglion cells animal model. One day before the beginning of the experiment, mice were daily intraperitoneal injected with minocycline (60 mg/kg, saline injected in the control group) till day 7, β3 tubulin positive cells and retinal GFAP protein expression were evaluated by tissue i mmol/L unofluorescence assay. Real-time PCR and Western blot assay were used to detect IFN-γ, IL-1, TNF-α and GFAP and vimentin mRNA and protein expression level in retinal tissues. Results Compared with control group, RGC survival rate in L-glutamate group was significantly reduced with a dose-and time-dependent. In addition,axon growth was inhibited with the treatment of glutamic acid, while these effects were abolished in the minocycline group ( F = 18.87, P < 0. 01 ). Animal study showed that the number of RGC dramatically decreased, however, expression of GFAP in retinal tissue significantly increased in L-glutamate treated mice,compared to control (F = 7.6, P < 0.01 ). Minocycline treatment significantly improved L-glutamate-induced ganglion cell damage and significantly reduced their GFAP expression. Both mRNA and protein expression levels of IFN-γ, IL-1, TNF-α and GFAP and Vimentin in retinal tissue of L-glutamic acid group significantly upregulated compared to control, while the minocycline significantly reduced the expression of these factors.Conclusions L-glutamic acid can induce retinal ganglion cells damage, inhibit axon growth and increase inflammatory and glial-cell related genes and proteins expression. Minocycline could significantly protect retinal ganglion cells from the injury caused by L-glutamate. Key words: Retinal ganglion cells; Cell death; Cytoprotection; Glutamic acid; Minocycline" @default.
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- W83035099 date "2010-09-01" @default.
- W83035099 modified "2023-09-24" @default.
- W83035099 title "[Protective effect of minocycline on glutamate-induced retinal ganglion cell injury and molecular mechanism]." @default.
- W83035099 doi "https://doi.org/10.3760/cma.j.issn.0412-4081.2010.09.008" @default.
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