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- W83049682 abstract "AIM: To generate an adenoviral vector carrying endothelial nitric-oxide synthase (eNOS) gene in order to mediate the expression of eNOS gene in gastrointestinal smooth muscle cells (SMC) and assess the enzyme activity of eNOS. METHODS: A recombinant adenovirus (Ad-eNOS) containing the bovine eNOS cDNA fragment was generated by homologous recombination in bacteria. The SMC of distal part of esophagus and gastric fundus of cat were isolated and cultured in vitro and infected with Ad-eNOS. The expression of eNOS gene was detected by Western blot and RT-PCR. The enzyme activity of NOS and the output of NO in SMC were measured by NOS and NO assay kit, furthermore, the different effects of given factors on the enzyme activity" @default.
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- W83049682 date "2003-07-15" @default.
- W83049682 modified "2023-09-27" @default.
- W83049682 title "Gastrointestinal smooth muscle cell as target for gene transfer of eNOS gene" @default.
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- W83049682 doi "https://doi.org/10.11569/wcjd.v11.i7.986" @default.
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