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• W830721725 abstract "This thesis is devoted to the study of mechanochemistry of single cells and single biomolecules. Mechanochemistry relates to the association of mechanical and chemical occurrence at the molecular level. Using a combination of microrheological measurements and Raman spectroscopy based on optical tweezers we have shown how the molecular structures of single living cells and biomolecules are coupled with mechanical deformations.&#x0D; The basic questions to respond in this work are: (i) How does a cell or a biomolecule detect an external force? (ii) How do the viscoelastic properties of living cells and biomolecules depend on its environment? (iii) How does the structure of the cell or biomolecules change because of the external force?&#x0D; We use microrheology to detect the response of single cells (or biomolecules) to an external force. Two point microrheology is applied to study these samples - both in their relax configuration and in a stress situation when a known external force is applied. We are able to find non-linearities in mechanical response of RBC and DNA molecules subjected to an external load. In both the cases, an increase in elastic stiffness of the system is observed with increase in the force.&#x0D; To detect changes in the molecular structures of DNA or a cell as a result of an applied mechanical load, Raman spectroscopy is used. Raman signals from biomolecules are rich and complex in nature and are accompanied with several noises. Hence processing of data is an important aspect in Raman spectroscopy. For gaining in-depth information regarding the behavior of the molecule under study, we used statistical techniques such as principal component analysis and 2D correlation spectroscopy.&#x0D; Raman measurement of single DNA, with excitation optical beam focused by conventional optics, is almost impossible owing to very small thickness of DNA and inherent low efficiency of Raman scattering. To overcome this difficulty, we used additional optical phenomena - Surface Enhanced Raman Scattering (SERS) technique. With implementation of SERS, we were able to detect signals from single DNA molecule. Our study presents substantial findings that describe the structural changes of the phosphate backbone when DNA is extended in the entropic force regime. The current belief is that such forces are balanced by lowering DNA entropy via unfolding as the molecule is extended. While this is accurate, we observe structural movement at the bond level when the single biomolecule is extended at these low forces. This revelation has never been measured directly or predicted theoretically. The result gives a different view of DNA mechanical properties. We confirm and substantiate our experimental results with molecular dynamics simulations." @default.
• W830721725 created "2016-06-24" @default.
• W830721725 creator A5005716717 @default.
• W830721725 date "2023-10-15" @default.
• W830721725 modified "2023-10-16" @default.
• W830721725 title "Mechanochemical study of single living cells and biomolecules combining optical tweezers and raman spectroscopy" @default.
• W830721725 cites W144262296 @default.
• W830721725 cites W1537061132 @default.
• W830721725 cites W1546843241 @default.
• W830721725 cites W1558524852 @default.
• W830721725 cites W1588835142 @default.
• W830721725 cites W1681122864 @default.
• W830721725 cites W1781054041 @default.
• W830721725 cites W1964441323 @default.
• W830721725 cites W1968286102 @default.
• W830721725 cites W1968686513 @default.
• W830721725 cites W1970592466 @default.
• W830721725 cites W1972354809 @default.
• W830721725 cites W1974915001 @default.
• W830721725 cites W1980256685 @default.
• W830721725 cites W1981569609 @default.
• W830721725 cites W1982376326 @default.
• W830721725 cites W1985150525 @default.
• W830721725 cites W1986766821 @default.
• W830721725 cites W1987215703 @default.
• W830721725 cites W1998276013 @default.
• W830721725 cites W1999173254 @default.
• W830721725 cites W2000040838 @default.
• W830721725 cites W2000355915 @default.
• W830721725 cites W2005943142 @default.
• W830721725 cites W2007199319 @default.
• W830721725 cites W2009186703 @default.
• W830721725 cites W2009827720 @default.
• W830721725 cites W2010334574 @default.
• W830721725 cites W2010739090 @default.
• W830721725 cites W2010889388 @default.
• W830721725 cites W2017312934 @default.
• W830721725 cites W2020409401 @default.
• W830721725 cites W2021448935 @default.
• W830721725 cites W2022817606 @default.
• W830721725 cites W2024067117 @default.
• W830721725 cites W2024316435 @default.
• W830721725 cites W2024377496 @default.
• W830721725 cites W2026669015 @default.
• W830721725 cites W2026831948 @default.
• W830721725 cites W2030181230 @default.
• W830721725 cites W2030261975 @default.
• W830721725 cites W2030335451 @default.
• W830721725 cites W2030365976 @default.
• W830721725 cites W2033540937 @default.
• W830721725 cites W2035014044 @default.
• W830721725 cites W2036012664 @default.
• W830721725 cites W2036371652 @default.
• W830721725 cites W2038447663 @default.
• W830721725 cites W2038729819 @default.
• W830721725 cites W2039610543 @default.
• W830721725 cites W2044024547 @default.
• W830721725 cites W2045812053 @default.
• W830721725 cites W2050512215 @default.
• W830721725 cites W2052204627 @default.
• W830721725 cites W2054523261 @default.
• W830721725 cites W2054635074 @default.
• W830721725 cites W2054737342 @default.
• W830721725 cites W2055280411 @default.
• W830721725 cites W2055566196 @default.
• W830721725 cites W2057351442 @default.
• W830721725 cites W2059579573 @default.
• W830721725 cites W2059681661 @default.
• W830721725 cites W2060519194 @default.
• W830721725 cites W2061125966 @default.
• W830721725 cites W2063311279 @default.
• W830721725 cites W2063397852 @default.
• W830721725 cites W2066550103 @default.
• W830721725 cites W2068436560 @default.
• W830721725 cites W2070168436 @default.
• W830721725 cites W2072223178 @default.
• W830721725 cites W2072443063 @default.
• W830721725 cites W2072720024 @default.
• W830721725 cites W2075927605 @default.
• W830721725 cites W2080722962 @default.
• W830721725 cites W2081085416 @default.
• W830721725 cites W2081586824 @default.
• W830721725 cites W2081767926 @default.
• W830721725 cites W2081858082 @default.
• W830721725 cites W2087100981 @default.
• W830721725 cites W2087249002 @default.
• W830721725 cites W2091273808 @default.
• W830721725 cites W2091588319 @default.
• W830721725 cites W2091928429 @default.
• W830721725 cites W2092380725 @default.
• W830721725 cites W2092842516 @default.
• W830721725 cites W2093012008 @default.
• W830721725 cites W2094033827 @default.
• W830721725 cites W2094938672 @default.
• W830721725 cites W2096203606 @default.
• W830721725 cites W2097293881 @default.
• W830721725 cites W2105490605 @default.
• W830721725 cites W2108461844 @default.
• W830721725 cites W2112746525 @default.
• W830721725 cites W2113372755 @default.

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