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• W83084174 abstract "SummaryPurple photosynthetic bacteria assimilate CO2 primarily via the Calvin reductive pentose phosphate pathway. Depending on the growth conditions, the amount of CO2 fixation required for growth and the level of Calvin cycle enzymes can vary considerably. Although numerous biochemical studies have demonstrated induction or derepression of the enzymes, the mechanisms underlying this control have remained elusive. Cloning and expression of Calvin cycle structural and regulatory genes from different photosynthetic bacteria have led to significant advances in our understanding of CO2 fixationat the molecular level. cbb genes have been shown to be clustered in several bacteria, including Rhodobacter sphaeroides, in which genes within two cbb gene clusters appear to be organized within operons thus coordinating expression at the level of transcription. Mutagenesis of cbb genes in Rb. sphaeroides has revealed independent and interdependent regulatory relationships between the two different operons that appear to be mediated through a common transcriptional regulatory protein, CbbR. Transcriptional regulation and cbbR genes have also been demonstrated in Chromatium vinosum and Rhodospirillum rubrum. Characterization of the CbbR proteins should provide clues to the identity of the molecular signal that serves as sensor of the environment to control expression of enzymes involved in CO2 assimilation. Alternate routes of CO2 fixationhave unexpectedly been exposed in Rubis CO deletion mutants constructed in Rb. sphaeroides and Rs. rubrum opening new avenues of research in CO2 fixation in the photosynthetic bacteria. The RubisCO mutants have also been used as host strains for analyzing foreign RubisCO enzymes. In Cm. vinosum, two distinct RubisCO coding sequences were identified by hybridization and expression in E. coli allowed characterization of both enzymes. Finally, nucleotide sequence comparisons of cbb structural and regulatory genes have raised provocative evolutionary questions concerning relationships between photosynthetic bacteria and other organisms." @default.
• W83084174 created "2016-06-24" @default.
• W83084174 creator A5056101592 @default.
• W83084174 date "2006-03-08" @default.
• W83084174 modified "2023-09-23" @default.
• W83084174 title "Genetic Analysis of CO2 Fixation Genes" @default.
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• W83084174 doi "https://doi.org/10.1007/0-306-47954-0_51" @default.
• W83084174 hasPublicationYear "2006" @default.
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