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- W830896881 abstract "This study was conducted to know characteristics of shikimate accumulation in various plants treated with glyphosate and to investigate a possibility for broadening application of the shikimate accumulation assay. The accumulation manner of shikimate dependent on the leaf age was different among plant species. When the shikimate accumulation assay with excised leaf tissue taken from 21 species of field- or greenhouse-grown plants was conducted, one to eightfold differences in shikimate levels exhibited with lower values in grasses than in broadleaves plants. Especially, shikimate accumulation began to occur at lower dose of glyphosate in grass plants. Leaf tissues excised from CP4-EPSPS-introduced soybean or potato did not accumulate shikimate even when treated with 80 and 64 ㎍ mL-1 glyphosate, respectively. On the other hand, when shikimate was determined in the whole plant treated with glyphosate, shikimate level was increased in a linear manner until 3 day after herbicide application with a higher accumulation than in the excised leaf tissues floated on glyphosate solution. At this case of determinating the shikimate levels in glyphosate-treated whole plant, a significant difference of shikimate amount was not observed even using distilled water or 1.25 N HCl solution instead of the 10 mM ammonium phosphate buffer for shikimate extraction. Taken together, this assay would be highly useful to identify and control glyphosate-resistant weeds in field, to develop glyphosate-resistant crops, and to distinguish glyphosate-resistant mechanisms, especially through supplement of any other assay techniques." @default.
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- W830896881 date "2008-01-01" @default.
- W830896881 modified "2023-09-23" @default.
- W830896881 title "Characteristics of Shikimate Accumulation in Various Plants Treated with Glyphsate" @default.
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