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• W833384089 abstract "AbstractTurnip yellows virus (TuYV) is one of the most significant viral diseases ofoilseed rape and may be one of main reasons why commercial oilseed rape crops do notreach their genetic potential. TuYV is transmitted by aphids, sap-sucking hemipteroidinsects, and the green peach aphid (GPA) is the predominant vector. TuYV can reduceoilseed rape yield by up to 26% in the UK and may also affect oil quality. Currentcontrol measures rely on insecticides; however, changing legislation and reducedeffectiveness necessitate novel approaches to virus control. In this thesis, the impact ofTuYV on the UK commercial oilseed rape crop was established and sources of partialresistance to TuYV and aphids were investigated. TuYV reduces yield and has a subtleimpact on seed physiology including small changes to fatty acid profiles andglucosinolate content. Furthermore, these changes appear to be genotype-dependent andnot as a result of virus accumulation in the plant. To learn more about TuYVtransmission by aphids, a novel, functional-genomics tool was developed to silenceaphid genes by plant-mediated RNA interference (PMRi). Highly specific proteininteractions between virus particles and aphid proteins are critical determinants ofcirculative transmission, a process whereby virus particles can move between aphid celllayers. However, the aphid components underlying these processes are poorlyunderstood. As the GPA Rack1 protein has been implicated in transcytosis of TuYVparticles across the aphid gut barrier, PMRi was used to dissect its role in the circulativetransmission process. This revealed that Rack1 may have a direct role in TuYVacquisition by GPA. This work further demonstrates the potential of PMRi as a postgenomicstool in aphids and similar insects, but also as a direct means of aphid and/orvirus control. These contrasting research strategies have provided a two-prongedapproach towards improving TuYV control." @default.
• W833384089 created "2016-06-24" @default.
• W833384089 creator A5066549539 @default.
• W833384089 date "2013-09-01" @default.
• W833384089 modified "2023-09-28" @default.
• W833384089 title "Control of turnip yellows virus : assessing impact on oilseed rape quality traits and dissecting circulative transmission by aphids" @default.
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