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- W83392051 abstract "Within the last few years, a set of synthetic riboswitches has been engineered, which expands the toolbox of genetic regulatory devices. Small molecule binding aptamers have been used for the design of such riboswitches by insertion into untranslated regions of mRNAs, exploiting the fact that upon ligand binding the RNA structure interferes either with translation initiation or pre-mRNA splicing in yeast. In combination with self-cleaving ribozymes, aptamers have been used to modulate RNA stability. In this chapter, we discuss the applicability of different aptamers, ways to identify novel genetic devices, the pros and cons of various insertion sites and the application of allosteric ribozymes. Our expertise help to apply synthetic riboswitches to engineer complex genetic circuits." @default.
- W83392051 created "2016-06-24" @default.
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- W83392051 date "2011-10-14" @default.
- W83392051 modified "2023-10-14" @default.
- W83392051 title "RNA-Based Networks: Using RNA Aptamers and Ribozymes as Synthetic Genetic Devices" @default.
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- W83392051 doi "https://doi.org/10.1007/978-1-61779-412-4_9" @default.
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