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- W83404417 abstract "Publisher Summary This chapter presents the approaches used to study the regulation of oxidative defense genes in Escherichia coli and Salmonella typhimurium . These general approaches should be useful in examining the response to oxidative stress in other organisms and in further elucidating the response in E. coli and S. typhimurium . The sensitivity of strains to oxidants has been assessed using several different approaches. The first evidence that E. coli cells possess a regulated defense response against oxidative stress came from growth curves of E. coli . AB11157 cultures grown in K medium to a density of 5 × 10 7 cells/ml were treated with 0, 10, and 30 μM hydrogen peroxide for 30 min. The cells were then challenged with a lethal dose of 5 mM hydrogen peroxide, and aliquots of cells were removed after 20, 40 and 60 rain, diluted, and spread on LB plates to determine the number of colony-forming units. The sensitivity or resistance of wild-type or mutant strains has also been determined by zone of inhibition assays. For these simple assays, 0.1 ml of an overnight culture is plated on an agar plate in 2 to 3 ml of soft agar and a disk impregnated with an oxidant is placed in the center of the agar. The zone of killing or inhibition surrounding the disk is then measured after 12 to 24 hr of growth. For this assay to be consistent the volume of the agar medium must be uniform." @default.
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- W83404417 date "1994-01-01" @default.
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- W83404417 title "Regulation of bacterial gene expression in response to oxidative stress" @default.
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- W83404417 doi "https://doi.org/10.1016/0076-6879(94)36017-0" @default.
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