FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W83413736> ?p ?o ?g. }

• W83413736 abstract "Mast cells are highly granulated cells of the immune system that upon stimulation release a number of inflammatory mediators including heparin and/or chondroitin sulphate (CS) proteoglycan (PG) and various heparin-binding proteases such as tryptase, chymase and carboxypeptidase A (CPA). Mast cell CPA is a zinc-metalloexopeptidase, cleaving substrates with carboxyl-terminal aliphatic or aromatic amino acids. In this thesis, the storage and activation of CPA was investigated, using bone marrow derived mast cells (BMMCs) from mice lacking heparin, either due to loss of the gene coding for the heparin biosynthesis enzyme, NDST-2, or the gene coding for the PG core protein serglycin (SG). We found that BMMCs from NDST-2-/- mice that thus lack heparin, but produce CS, lack the chymase, mMCP-5 and mature CPA. Interestingly, the pro-form, but not the active form of CPA could be detected in the heparin-deficient cells, indicating a role for heparin in the processing of CPA. Furthermore, we have shown that the cysteine proteases, cathepsins C and S, are not involved in processing CPA, but rather that lack of cathepsin C or S cause increased levels of CPA as well as mMCP-5. In addition, neither cathepsins B nor L influence CPA processing at all, but instead, an aspartic protease, cathepsin E, plays a role in processing pro-CPA. Further, these studies led to the novel finding that cathepsin E is located inside the mast cell granules, where it is stored in complex with heparin. The activation of mast cells, which ultimately leads to degranulation, has been studied in detail; however, the process where mast cell granules are formed has not gained as much attention. We addressed this issue by the use of BMMCs from mice lacking SG. Here, we present evidence that secretory granules are formed independently of SG PG but that SG mediates selective condensation of certain granule constituents, while others are independent of SG. Mast cell proteases are correctly sorted into the granules but are subsequently degraded, exocytosed or remain unprocessed when SG is absent. These results indicate a model in which selected granule constituents are sorted into granules by SG-mediated retention." @default.
• W83413736 created "2016-06-24" @default.
• W83413736 creator A5004646639 @default.
• W83413736 date "2005-08-01" @default.
• W83413736 modified "2023-09-24" @default.
• W83413736 title "Mast cell carboxypeptidase A, a secretory granule component" @default.
• W83413736 cites W100325461 @default.
• W83413736 cites W121317997 @default.
• W83413736 cites W132399929 @default.
• W83413736 cites W1483162601 @default.
• W83413736 cites W1489386260 @default.
• W83413736 cites W1496451487 @default.
• W83413736 cites W1504566925 @default.
• W83413736 cites W1507938571 @default.
• W83413736 cites W1511722376 @default.
• W83413736 cites W1515314521 @default.
• W83413736 cites W1519269060 @default.
• W83413736 cites W1528764883 @default.
• W83413736 cites W1529162605 @default.
• W83413736 cites W1534120898 @default.
• W83413736 cites W1554177661 @default.
• W83413736 cites W1555703707 @default.
• W83413736 cites W1563664354 @default.
• W83413736 cites W1568439447 @default.
• W83413736 cites W1572929810 @default.
• W83413736 cites W1572955611 @default.
• W83413736 cites W1574017432 @default.
• W83413736 cites W1577884079 @default.
• W83413736 cites W1582826730 @default.
• W83413736 cites W1586061801 @default.
• W83413736 cites W1587223545 @default.
• W83413736 cites W1596543212 @default.
• W83413736 cites W1599496006 @default.
• W83413736 cites W1607493519 @default.
• W83413736 cites W1607581180 @default.
• W83413736 cites W1636878974 @default.
• W83413736 cites W1647944260 @default.
• W83413736 cites W1680126513 @default.
• W83413736 cites W1680932833 @default.
• W83413736 cites W1721611529 @default.
• W83413736 cites W1751541220 @default.
• W83413736 cites W178946232 @default.
• W83413736 cites W1828661048 @default.
• W83413736 cites W1845210287 @default.
• W83413736 cites W1859951310 @default.
• W83413736 cites W1871640811 @default.
• W83413736 cites W1917722907 @default.
• W83413736 cites W1930831294 @default.
• W83413736 cites W1936798053 @default.
• W83413736 cites W1946297344 @default.
• W83413736 cites W1955765029 @default.
• W83413736 cites W1959914147 @default.
• W83413736 cites W1965630231 @default.
• W83413736 cites W1966400525 @default.
• W83413736 cites W1966472906 @default.
• W83413736 cites W1969525788 @default.
• W83413736 cites W1971846568 @default.
• W83413736 cites W1972140057 @default.
• W83413736 cites W1973157062 @default.
• W83413736 cites W1973602059 @default.
• W83413736 cites W1974468764 @default.
• W83413736 cites W1975068237 @default.
• W83413736 cites W1975996690 @default.
• W83413736 cites W1976229113 @default.
• W83413736 cites W1977260572 @default.
• W83413736 cites W1980273234 @default.
• W83413736 cites W1980727190 @default.
• W83413736 cites W1981930064 @default.
• W83413736 cites W1982709334 @default.
• W83413736 cites W1983746522 @default.
• W83413736 cites W1984583241 @default.
• W83413736 cites W1984988773 @default.
• W83413736 cites W1986738891 @default.
• W83413736 cites W1990860772 @default.
• W83413736 cites W1994617732 @default.
• W83413736 cites W1995103492 @default.
• W83413736 cites W1998356424 @default.
• W83413736 cites W2001175660 @default.
• W83413736 cites W2002600941 @default.
• W83413736 cites W2003293767 @default.
• W83413736 cites W2003909751 @default.
• W83413736 cites W2006373098 @default.
• W83413736 cites W2007945620 @default.
• W83413736 cites W2014623953 @default.
• W83413736 cites W2022795264 @default.
• W83413736 cites W2023926028 @default.
• W83413736 cites W2028396701 @default.
• W83413736 cites W2029389633 @default.
• W83413736 cites W2034237238 @default.
• W83413736 cites W2038004252 @default.
• W83413736 cites W2039908865 @default.
• W83413736 cites W2045341219 @default.
• W83413736 cites W2045577652 @default.
• W83413736 cites W2046603954 @default.
• W83413736 cites W2047376205 @default.
• W83413736 cites W2047560583 @default.
• W83413736 cites W2049197565 @default.
• W83413736 cites W2049371451 @default.
• W83413736 cites W2049796670 @default.
• W83413736 cites W2052171579 @default.

• next