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- W836256 abstract "A modified Langendorff heart preparation was designed for use with a 4.7 Tesla Nicolet NMR spectrometer operated at 80.98 MHz in the pulsed Fourier transform mode. The perfusion system delivered constant temperature (37/sup 0/C) modified Krebs-Henseleit solution containing glucose as the exogenous substrate. Experiments were performed with constant perfusion pressure, which was adjusted according to the age of the rabbit. Neonatal hearts weighted about 0.5 g. A special 16mm Dadok-type NMR probe was designed to accommodate these small hearts in a vertical bore spectrometer configuration. Spectral standardization was accomplished with a capillary tube placed in the B1 window of the probe. The principal theme of the experiments was to determine the effects of ischemic stress on the neonatal heart by measuring changes in intracellular pH, inorganic phosphate (Pi), phosphocreatine (PCr) and ATP from 31-phosphorus spectra gathered over five minute intervals by signal averaging 148 FIDs using 4K data files and 5000 Hz spectral width. Hearts were subjected to different durations of ischemic stress. The responses of neonatal hearts were compared with those of more mature hearts." @default.
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- W836256 date "1986-03-05" @default.
- W836256 modified "2023-09-28" @default.
- W836256 title "31-Phosphorus nuclear magnetic resonance study of neonatal myocardial metabolism" @default.
- W836256 hasPublicationYear "1986" @default.
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