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- W837343517 abstract "This chapter discusses one potential approach involving genomic characterization of the naturally occurring microbial genomes, without the requirement of isolating individual microbes one by one in pure culture. It describes some of the methodological options and considerations, and presents a few of the protocols that have been used successfully for preparing genomic libraries from natural microbial assemblages. Some of the earliest large-fragment genome libraries from natural microbial populations made use of bacteriophage lambda cloning protocols. Today, advanced vector systems such as fosmids and bacterial artificial chromosomes (BACs) are now in wide use. BAC and fosmid libraries are useful tools that can be used to sidestep many thorny problems that still inhibit the accurate characterization of naturally occurring microbial communities. They represent useful tools for conducting sequence surveys to determine the phylogenetic and functional content and properties inherent in native microbial populations. They can also serve as reagents for gene discovery, gene expression, and functional genomic characterization of naturally occurring microbes, some still resistant to cultivation techniques. BAC and fosmid libraries can also serve as useful reagents in microarray construction and analyses of naturally occurring microbial dynamics and variability." @default.
- W837343517 created "2016-06-24" @default.
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- W837343517 date "2014-04-30" @default.
- W837343517 modified "2023-09-24" @default.
- W837343517 title "Construction of BAC and Fosmid Libraries from Naturally Occurring Microbial Populations" @default.
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- W837343517 doi "https://doi.org/10.1128/9781555817497.ch38" @default.
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