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- W83740709 abstract "The discoveries of genetic exchange systems for two members of the genus Rhodopseudomonas have increased the potential usefulness of biochemical genetics approaches to research with photosynthetic bacteria. This chapter presents four techniques for the isolation of mutant rhodopseudomonads. These include two variations of the basic penicillin selection, one adapted for Rhodopseudomonas sphaeroides , which is relatively resistant to penicillin, and the other that has been very useful with Rhodopseudomonas capsulata and involves solid media. The third technique described in this chapter uses cycloserine as a selective agent; although this might be expected a priori to function much as a penicillin selection, an unexpectedly interesting collection of survivors are typically obtained. Cycloserine does not inhibit the growth of Rhodopseudomonas capsulata , thus the technique is not applicable to that species. The fourth technique selects for mutants defective in photosynthetically driven active transport and may be termed “tetracycline suicide.” It is based on the observation of light-induced tetracycline accumulation by a phototrophic bacterium." @default.
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- W83740709 date "1980-01-01" @default.
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- W83740709 title "[3] Isolation of mutants of photosynthetic bacteria" @default.
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- W83740709 doi "https://doi.org/10.1016/s0076-6879(80)69005-3" @default.
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