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- W83833467 abstract "Human Cytomegalovirus (HCMV), one of the herpesviruses, is medically significant both as a cause of birth defects and as a source of problems in immunosuppressed individuals. Because the virus is highly cell-associated, it is difficult to obtain large quantities of pure viral DNA. For this reason we have sought to construct a cloned library of the HCMV genome to aid in further studies of the molecular biology of HCMV infections. The cloning was accomplished as follows: Eco R1 fragments of the AD169 strain of HCMV (0.27 μg of DNA) were inserted into the Eco R1 site of the E. coli plasmid PACYC 184. Approximately 9,000 colonies were selected on the basis of tetracycline resistance and chloramphenicol sensitivity. After isolation and Eco R1 cleavage of the recombinant plasmids, the cloned fragments were analyzed for co-migration on agarose gels with authentic Eco R1 digested HCMV DNA. The viral origin of the fragments was determined by hybridization of 32P CMV DNA to Southern blots of the inserts. In those cases where multiple clones containing the same size fragment were obtained, the DNA was cleaved with additional restriction endonucleases to characterize further the insert. To date, we have analyzed 84 clones and the viral inserts obtained represent at least 80% of the genome." @default.
- W83833467 created "2016-06-24" @default.
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- W83833467 date "1980-01-01" @default.
- W83833467 modified "2023-09-24" @default.
- W83833467 title "MOLECULAR CLONING OF THE HUMAN CYTOMEGALOVIRUS GENOME (STRAIN AD169)" @default.
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- W83833467 doi "https://doi.org/10.1016/b978-0-12-255850-4.50008-1" @default.
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