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- W839530771 startingPage "1847" @default.
- W839530771 abstract "The uptake and metabolism of methotrexate were examined in Crithidia fasciculata ATCC 12857. When the cells grown in a medium containing, biopterin were incubated with methotrexate, the uptake of methotrexate by the cells was completed within 1 min, and the amounts of methotrexate per 108 cells were maintained between 19 and 37 fmol/min throughout the remaining growth period. In contrast, the uptake of folate was linear for at least 20 min, and the uptake rate of folate by the cells varied from 31 to 247 fmol/min/108 cells during the growth. The folate uptake was inhibited by methotrexate and aminopterin. When C. fasciculata was cultivated in a medium containing methotrexate, the methotrexate taken up by the cells was metabolized to several compounds. One of the metabolites was isolated from the cells and the culture supernatant, using DEAE-cellulose and Sephadex G-10 column chromatographies, and identified as 4-amino-4-deoxy-10-methylpteroic acid (AMPte) on the basis of its behavior on thin-layer chromat..." @default.
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- W839530771 date "1983-01-01" @default.
- W839530771 modified "2023-10-03" @default.
- W839530771 title "Metabolism of folate and related compounds in a trypanosomid flagellate, Crithidia fasciculata. Part V. Uptake and metabolism of methotrexate by Crithidia fasciculata." @default.
- W839530771 cites W1867106612 @default.
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- W839530771 doi "https://doi.org/10.1271/bbb1961.47.1847" @default.
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