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• W840725479 abstract "한국 분리주 감자잎말림바이러스 (PLRV)로부터 627bp 크기의 외피단백질 유전자의 ORF (AF296280)를 분리하여 장려품종 감자인 '수미'를 형질전환 하였다. 17계통의 형질전환체를 선발하여 온실과 포장에서 5세대를 증식하면서 PLRV에 대한 저항성이 큰 5계통을 선발 하였다. 도입된 유전자들의 유전적인 안정성을 확인하기위해 PCR, Southern, 그리고 northern blot 분석을 수행하였다. 또한 클론으로 증식된 형질전환 감자들의 특성과 저항성도 검정하였다. 형질전환된 감자들에서 PLRV의 외피단백질 유전자는 안전적으로 발현되며 저항성을 유지하였으며, 유사도가 비교적 낮은 감자 바이러스 Y (PVY)에는 저항성을 나타내지 않았다. 따라서 이러한 저항성은 도입된 유전자와 유사성도가 높은 바이러스에 저항성을 나타내는 homology dependent gene silencing으로 판단되었다. 유망계통 형질전환 감자 계통들의 포장평가를 통해 PLRV에 대한 저항성을 제외한 주요한 농업적 특성과 식물학적 특성은 형질전환 되지 않은 감자와 큰 차이를 보이지 않았다. The coat protein gene (AF296280) of the Korean isolate Potato leafroll virus (PLRV) was cloned and the open reading frame (627 bp) was transformed into potato (Solanum tuberosum cv. Superior). Out of seventeen individual transgenic lines, five lines were identified to confer resistance to PLRV through the five generation's selection program in the greenhouse as well as isolated trial field. Successful introduction and genetic stability of coat protein gene in the genome of potato were confirmed by polymerase chain reaction (PCR), Southern blot hybridization and northern blot hybridization. Some of the transgenic lines were highly resistant to PLRV but did not show any resistance to less homologous Potato virus Y (PVY). Our results suggest that the resistance to PLRV is due to homology dependent gene silencing by sense strand coat protein gene. In addition, the results of field test through five generations showed that there were no significant differences comparing to nontransgenic potatoes in the morphological aspect of shoot as well as tuber, Ho remarkable differences were also observed in the major agronomic characters and yields except for the resistance to PLRV." @default.
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• W840725479 date "2005-12-01" @default.
• W840725479 modified "2023-10-18" @default.
• W840725479 title "Cloning of Coat Protein Gene from Korean Isolate Potato Leafroll Virus (PLRV) and Introduction into Potato (Solanum tuberosum)" @default.
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• W840725479 doi "https://doi.org/10.5010/jpb.2005.32.4.243" @default.
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