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- W841133811 abstract "RolB gene (∼ 800 bp) was isolated from Agrobacterium rhizogenes in PCR with rolB5kod-rolB3kod primers. The identity of gene was confirmed by sequence analysis. Binary constructs basing on pART27 and pNOV2819 plasmids and containing rolB gene under (a) own promoter sequence, (b) 35S CaMV, and (c) stress-induced PR promoter were prepared and used to transform quince (Cydonia oblonga). 1992 explants derived from clones K.I, K.16 and K.19 were transformed using A. tumefaciens. The efficiency of plant regeneration was strongly dependent on concentration of TDZ, and was best with 10 and 32 μM TDZ. The highest number of regenerating untransformed plants was obtained from explants of K.ll (43.8%), meanwhile after transformation the best results of regeneration was observed for explants derived from K.16 (from 22.2% to 2.78% dependent on type of construct). Rooting was observed in plants regenerated from explants transformed with plasmid pART27rolBdir3." @default.
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- W841133811 date "2006-01-01" @default.
- W841133811 modified "2023-10-13" @default.
- W841133811 title "Transformation of quince (Cydonia oblonga) with the rolB gene-based constructs under different promoters" @default.
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