FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W84204737> ?p ?o ?g. }

• W84204737 endingPage "295" @default.
• W84204737 startingPage "295" @default.
• W84204737 abstract "To study the effect of a novel targeted ribonuclease (TN), the fusion protein of HBVc and human eosinophil-derived neurotoxin (hEDN), on the HBV replication in vitro.The gene encoding the targeted ribonuclease was cloned into pcDNA3.1 (-) to form recombinant eukaryotic expression vector p/TN. Control plasmids, including p/hEDN, p/HBVc, and p/TNmut in which a Lys113-Arg mutation was introduced by sequential PCR to eliminate the ribonuclease activity of hEDN, were also constructed. Liposome-mediated transfection of 2.2.15 cells by p/TN, p/TNmut, p/hEDN, p/HBVc, and pcDNA3.1 (-), or mock transfection was performed. After that, RT-PCR was used to verify the transgene expression. Morphology of the transfected cells was observed and MTT assay was performed to detect the cytotoxicity of transgene expression. Concentration of HBsAg in the supernatant of the transfected cells was measured using solid-phase radioimmunoassay.Transgenes were successfully expressed in 2.2.15 cells. No obvious cytotoxic effect of transgene expression on 2.2.15 cells was found. The HBsAg concentration in the p/TN transfected cells was reduced by 58 % compared with that of mock transfected cells. No such an effect was found in all other controls.The targeted ribonuclease can inhibit HBV replication in vitro while it has no cytotoxicity on host cells. The targeted ribonuclease may be used as a novel antiviral agent for human HBV infection." @default.
• W84204737 created "2016-06-24" @default.
• W84204737 creator A5006776778 @default.
• W84204737 creator A5027748236 @default.
• W84204737 creator A5027835055 @default.
• W84204737 creator A5066602086 @default.
• W84204737 creator A5072795022 @default.
• W84204737 creator A5074193127 @default.
• W84204737 creator A5080687802 @default.
• W84204737 date "2003-01-01" @default.
• W84204737 modified "2023-09-23" @default.
• W84204737 title "Targeted ribonuclease can inhibit replication of hepatitis B virus" @default.
• W84204737 cites W1501417543 @default.
• W84204737 cites W1540094841 @default.
• W84204737 cites W1832237372 @default.
• W84204737 cites W1894278338 @default.
• W84204737 cites W1962682498 @default.
• W84204737 cites W1963730658 @default.
• W84204737 cites W1964896501 @default.
• W84204737 cites W1967159039 @default.
• W84204737 cites W1967539989 @default.
• W84204737 cites W1983064802 @default.
• W84204737 cites W1990771267 @default.
• W84204737 cites W1991046721 @default.
• W84204737 cites W1992194872 @default.
• W84204737 cites W1994901499 @default.
• W84204737 cites W2002504656 @default.
• W84204737 cites W2007902841 @default.
• W84204737 cites W2010338556 @default.
• W84204737 cites W2025726431 @default.
• W84204737 cites W2028380992 @default.
• W84204737 cites W2029447838 @default.
• W84204737 cites W2039813523 @default.
• W84204737 cites W2047908387 @default.
• W84204737 cites W2055270948 @default.
• W84204737 cites W2056767522 @default.
• W84204737 cites W2063330692 @default.
• W84204737 cites W2073213047 @default.
• W84204737 cites W2076240008 @default.
• W84204737 cites W2078957262 @default.
• W84204737 cites W2079659749 @default.
• W84204737 cites W2085990351 @default.
• W84204737 cites W2091317905 @default.
• W84204737 cites W2097074859 @default.
• W84204737 cites W2107057287 @default.
• W84204737 cites W2108860654 @default.
• W84204737 cites W2136497869 @default.
• W84204737 cites W2150001528 @default.
• W84204737 cites W2158896168 @default.
• W84204737 cites W2291407818 @default.
• W84204737 cites W2318639814 @default.
• W84204737 cites W2381512981 @default.
• W84204737 cites W2444664927 @default.
• W84204737 cites W4255167765 @default.
• W84204737 doi "https://doi.org/10.3748/wjg.v9.i2.295" @default.
• W84204737 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4611332" @default.
• W84204737 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12532452" @default.
• W84204737 hasPublicationYear "2003" @default.
• W84204737 type Work @default.
• W84204737 sameAs 84204737 @default.
• W84204737 citedByCount "25" @default.
• W84204737 countsByYear W842047372012 @default.
• W84204737 countsByYear W842047372013 @default.
• W84204737 countsByYear W842047372014 @default.
• W84204737 countsByYear W842047372015 @default.
• W84204737 countsByYear W842047372016 @default.
• W84204737 countsByYear W842047372021 @default.
• W84204737 countsByYear W842047372022 @default.
• W84204737 crossrefType "journal-article" @default.
• W84204737 hasAuthorship W84204737A5006776778 @default.
• W84204737 hasAuthorship W84204737A5027748236 @default.
• W84204737 hasAuthorship W84204737A5027835055 @default.
• W84204737 hasAuthorship W84204737A5066602086 @default.
• W84204737 hasAuthorship W84204737A5072795022 @default.
• W84204737 hasAuthorship W84204737A5074193127 @default.
• W84204737 hasAuthorship W84204737A5080687802 @default.
• W84204737 hasBestOaLocation W842047371 @default.
• W84204737 hasConcept C102230213 @default.
• W84204737 hasConcept C104317684 @default.
• W84204737 hasConcept C109316439 @default.
• W84204737 hasConcept C153911025 @default.
• W84204737 hasConcept C154317977 @default.
• W84204737 hasConcept C159047783 @default.
• W84204737 hasConcept C202751555 @default.
• W84204737 hasConcept C2522874641 @default.
• W84204737 hasConcept C2777410769 @default.
• W84204737 hasConcept C2778976237 @default.
• W84204737 hasConcept C2780593183 @default.
• W84204737 hasConcept C54009773 @default.
• W84204737 hasConcept C54355233 @default.
• W84204737 hasConcept C55493867 @default.
• W84204737 hasConcept C67705224 @default.
• W84204737 hasConcept C81885089 @default.
• W84204737 hasConcept C86803240 @default.
• W84204737 hasConceptScore W84204737C102230213 @default.
• W84204737 hasConceptScore W84204737C104317684 @default.
• W84204737 hasConceptScore W84204737C109316439 @default.
• W84204737 hasConceptScore W84204737C153911025 @default.

• next