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• W842237599 abstract "Research Article1 December 1991free access Requirement of a polypyrimidine tract for trans-splicing in trypanosomes: discriminating the PARP promoter from the immediately adjacent 3′ splice acceptor site. J. Huang J. Huang Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032. Search for more papers by this author L.H. Van der Ploeg L.H. Van der Ploeg Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032. Search for more papers by this author J. Huang J. Huang Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032. Search for more papers by this author L.H. Van der Ploeg L.H. Van der Ploeg Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032. Search for more papers by this author Author Information J. Huang1 and L.H. Van der Ploeg1 1Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032. The EMBO Journal (1991)10:3877-3885https://doi.org/10.1002/j.1460-2075.1991.tb04957.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info We studied sequence requirements for trans-splicing at the 3′ splice acceptor site of a procyclic acidic repetitive protein (PARP) coding gene in trypanosomes. In transient CAT transfection assays with linker scanning (LS) mutants in a PARP promoter--3′ splice acceptor site--CAT construct, minor differences in the sequence composition of the polypyrimidine tract (nt −36 to −5 with respect to the 3′ splice acceptor site) severely affected the CAT activity. Analysis of steady-state CAT RNA in stably transformed trypanosomes revealed that the LS mutations had indeed affected the pre-mRNA splicing efficiency. The data indicate that mini-exon addition is not required simply for maturation of polycistronic pre-mRNA but is also essential for the generation of functional mRNA from monocistronic genes, since unspliced monocistronic pre-mRNA did not accumulate or allow synthesis of CAT. We postulate that mini-exon addition at polycistronically transcribed genes, which can have drastically different polypyrimidine tracts at each of their 3′ splice acceptor sites, can occur with different efficiencies for each gene of the array thus affecting mRNA abundance. Previous ArticleNext Article Volume 10Issue 121 December 1991In this issue RelatedDetailsLoading ..." @default.
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• W842237599 date "1991-12-01" @default.
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• W842237599 title "Requirement of a polypyrimidine tract for trans-splicing in trypanosomes: discriminating the PARP promoter from the immediately adjacent 3′ splice acceptor site." @default.
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• W842237599 doi "https://doi.org/10.1002/j.1460-2075.1991.tb04957.x" @default.
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