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• W84228304 abstract "RNA interference (RNAi) is sequence-specific inhibition of gene expression induced by double-stranded RNA.Define the role of Wilms tumor 1 gene (WT1) in breast cancer oncogenesis using RNAi.MCF-7 breast cancer cells, which express a high level of WT1, were transfected with synthetic small interfering RNA (siRNA) targeting WT1 (siRNA(WT1)) resulting in inhibition of WTI expression, as well as growth, in a dose- and time-dependent manner.The minimum concentration of siRNA(WT1) for growth inhibition and WT1 silencing was 25 nM and 50 nM respectively. WT1 expression was completely abolished at 200 nM siRNA,.These data suggest that WTI1is indispensable for the survival of breast cancer MCF-7 cell line." @default.
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• W84228304 date "2007-11-01" @default.
• W84228304 modified "2023-09-23" @default.
• W84228304 title "Growth inhibition of breast cancer cell line MCF-7 by siRNA silencing of Wilms tumor 1 gene." @default.
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