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- W84263886 abstract "AbstractCo-affinity purification methods can test whether two proteins physically engage in a complex. The assay principle is to enrich cellular extracts for a first protein by a purification step, and then test if a second protein is enriched as well. This principle has been optimized for use at high-throughput in LUMIER (luminescence-based mammalian interactome mapping) assays, which use luciferase-tags for the detection of the second proteins. This protocol is ideal for miniaturization of the assay on microtiter plates and supports a throughput of several hundred interaction tests per week.Key wordsProtein–protein interactionsLuciferaseProtein networksHigh-throughput screensInteractome" @default.
- W84263886 created "2016-06-24" @default.
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- W84263886 date "2013-01-01" @default.
- W84263886 modified "2023-09-26" @default.
- W84263886 title "Analysis of Protein–Protein Interactions Using LUMIER Assays" @default.
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- W84263886 doi "https://doi.org/10.1007/978-1-62703-601-6_2" @default.
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