FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W84413> ?p ?o ?g. }

• W84413 abstract "Every living organism has been equipped with some form of barrier, separating the body from the environment. Its complexity depends on the degree of protection required from physical, mechanical and chemical insults. In humans this function is performed by the skin, which covers the outside of the body, protecting inner cells and organs. The outermost layer of the skin, the epidermis, is a continuously regenerating tissue as its cells are lost to normal wear and tear. This process, called terminal or epidermal differentiation, is highly organised and involves the tightly controlled expression of many specific proteins. The most predominant cells of the epidermis, the keratinocytes, arise from the stem cells that line the basement membrane and pass outwards through a series of differentiation stages, towards the surface of the skin where they become a part of the cornified cell envelope, from which they are eventually sloughed into the environment. Barrier function and the renewal of the epidermis are highly dependent on the individual genes, their protein products and interaction.One of the most predominant protein markers of epidermal differentiation is filaggrin. It is a product of proteolytic processing of the large precursor protein, profilaggrin, and has been shown to interact with keratin filaments to form highly ordered structures known as macrofibrils. In later stages of the epidermal differentiation individual filaggrin repeats undergo further post-translational modifications and are proteolysed into free amino acids that may be involved both in protection against ultraviolet light and in the water-holding capacity of the skin. This thesis primarily examines a novel repetitive protein identified in mouse epidermis that has high homology to the human and mouse profilaggrin genes. The chromosomal localisation, repetitive structure, amino acid composition and expression studies suggest that this protein is involved in the late stages of the epidermal differentiation and cornified cell envelope formation. In a mouse individual repeats are of 74-80 residues with each repeat having a pi of between 8.0 and 11.6 and are rich in proline, glycine, serine, glutamine and arginine residues. The amino terminus, as in profilaggrin, contains two calcium-binding motifs of the EF-hand type with a high homology to the SI00 proteins. Northern analysis revealed that the gene is expressed predominantly in the epidermis, with RT PCR detecting very low abundance in the tongue. The gene has been mapped to the epidermal differentiation complex (EDC) region on chromosome 3, 20 kb upstream of the profilaggrin gene. Blast searches of the available human and mouse genomes suggest that this is the last filaggrin-like protein containing EF-hand calcium binding domain. A polyclonal antibody produced, detects high molecular weight products as well as putative processed products in western blots of epidermal extracts. Double-label immunofluorescence localised the novel protein to the more differentiated cells of the granular layer and stratum corneum.Functionally the results show that this protein is similar to filaggrin; this protein assembles keratin filaments into macrofibrils in vitro and binds calcium. The processed repeats rich in arginine, glutamine, glutamic acid and histidine are potential sources of these amino acids for the integral processes involved in proteolytic breakdown of macrofibrils, hydration of the stratum comeum and UV protection. Additional mapping studies have identified the human homolog that has been located on chromosome lq21, the syntenic region of mouse chromosome 3. Based on homology data, the repetitive structure and amino acid composition, it has been proposed to name this protein filaggrin related protein (Flg-2)." @default.
• W84413 created "2016-06-24" @default.
• W84413 creator A5059213218 @default.
• W84413 date "2003-01-01" @default.
• W84413 modified "2023-09-24" @default.
• W84413 title "Characterisation of the epidermal differentiation complex (EDC) on mouse chromosome 3 and human chromosome 1q21 : characterisation of Flg-2, a novel epidermal protein that is abundantly expressed in mammalian epidermis and functionally related to filaggri" @default.
• W84413 hasPublicationYear "2003" @default.
• W84413 type Work @default.
• W84413 sameAs 84413 @default.
• W84413 citedByCount "0" @default.
• W84413 crossrefType "journal-article" @default.
• W84413 hasAuthorship W84413A5059213218 @default.
• W84413 hasConcept C104317684 @default.
• W84413 hasConcept C105702510 @default.
• W84413 hasConcept C108335385 @default.
• W84413 hasConcept C142669718 @default.
• W84413 hasConcept C148738053 @default.
• W84413 hasConcept C1491633281 @default.
• W84413 hasConcept C190684412 @default.
• W84413 hasConcept C202751555 @default.
• W84413 hasConcept C203014093 @default.
• W84413 hasConcept C2776008674 @default.
• W84413 hasConcept C2776391296 @default.
• W84413 hasConcept C2776458125 @default.
• W84413 hasConcept C2777074287 @default.
• W84413 hasConcept C2777577635 @default.
• W84413 hasConcept C2778329239 @default.
• W84413 hasConcept C54355233 @default.
• W84413 hasConcept C78383274 @default.
• W84413 hasConcept C81241287 @default.
• W84413 hasConcept C86803240 @default.
• W84413 hasConcept C95444343 @default.
• W84413 hasConceptScore W84413C104317684 @default.
• W84413 hasConceptScore W84413C105702510 @default.
• W84413 hasConceptScore W84413C108335385 @default.
• W84413 hasConceptScore W84413C142669718 @default.
• W84413 hasConceptScore W84413C148738053 @default.
• W84413 hasConceptScore W84413C1491633281 @default.
• W84413 hasConceptScore W84413C190684412 @default.
• W84413 hasConceptScore W84413C202751555 @default.
• W84413 hasConceptScore W84413C203014093 @default.
• W84413 hasConceptScore W84413C2776008674 @default.
• W84413 hasConceptScore W84413C2776391296 @default.
• W84413 hasConceptScore W84413C2776458125 @default.
• W84413 hasConceptScore W84413C2777074287 @default.
• W84413 hasConceptScore W84413C2777577635 @default.
• W84413 hasConceptScore W84413C2778329239 @default.
• W84413 hasConceptScore W84413C54355233 @default.
• W84413 hasConceptScore W84413C78383274 @default.
• W84413 hasConceptScore W84413C81241287 @default.
• W84413 hasConceptScore W84413C86803240 @default.
• W84413 hasConceptScore W84413C95444343 @default.
• W84413 hasLocation W844131 @default.
• W84413 hasOpenAccess W84413 @default.
• W84413 hasPrimaryLocation W844131 @default.
• W84413 hasRelatedWork W1591652724 @default.
• W84413 hasRelatedWork W1825657012 @default.
• W84413 hasRelatedWork W1966219402 @default.
• W84413 hasRelatedWork W1968210913 @default.
• W84413 hasRelatedWork W1980256159 @default.
• W84413 hasRelatedWork W2037409208 @default.
• W84413 hasRelatedWork W2050412379 @default.
• W84413 hasRelatedWork W2063702640 @default.
• W84413 hasRelatedWork W2064302574 @default.
• W84413 hasRelatedWork W2083324425 @default.
• W84413 hasRelatedWork W2084327894 @default.
• W84413 hasRelatedWork W2086023112 @default.
• W84413 hasRelatedWork W2086803868 @default.
• W84413 hasRelatedWork W2087808531 @default.
• W84413 hasRelatedWork W2148317031 @default.
• W84413 hasRelatedWork W2161700314 @default.
• W84413 hasRelatedWork W2413686150 @default.
• W84413 hasRelatedWork W315929630 @default.
• W84413 hasRelatedWork W2825144662 @default.
• W84413 hasRelatedWork W3152279617 @default.
• W84413 isParatext "false" @default.
• W84413 isRetracted "false" @default.
• W84413 magId "84413" @default.
• W84413 workType "article" @default.