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- W846099033 abstract "Defensins are antifungal peptides contributing to innate immunity in plants and animals. In this study, we characterized an Arabidopsis defensin gene PDF1.1 in detail. Northern blot analysis showed maximal accumulation of PDF1.1 gene transcripts 8 h after methyl jasmonate treatment on rosette leaves. Expression of the gene was induced by methyl jasmonate and ethylene treatments, exhibiting synergistic effect between the two volatile phytohormones. The gene was fused to CaMV35S promoter and transformed into Arabidopsis to generate transgenic plants over-expressing the gene. Successful transformants were selected on antibiotics-containing medium. Lines integrated with single copy of the transgene were further selected by genomic Southern blot analysis. The transgenic plants exhibited enhanced resistance to fungal pathogen Alternaria brassicicola, illustrating jasmonate-dependent defense response." @default.
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- W846099033 date "2004-09-01" @default.
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- W846099033 title "Enhanced Disease-resistance of Transgenic Arabidopsis Over-expressing the Defensin Gene PDF1.1" @default.
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