FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W847075346> ?p ?o ?g. }

• W847075346 abstract "In most mammals, extended inactivity or immobilisation of skeletal muscle (e.g. bedrest, limb-casting or hindlimb unloading) results in muscle disuse atrophy, a process which is characterised by the loss of skeletal muscle mass and function. In stark contrast, animals that experience natural bouts of prolonged muscle inactivity, such as hibernating mammals and aestivating frogs, consistently exhibit limited or no change in either skeletal muscle size or contractile performance. While many of the factors regulating skeletal muscle mass are known, little information exists as to what mechanisms protect against muscle atrophy in some species. Green-striped burrowing frogs (Cyclorana alboguttata) survive in arid environments by burrowing underground and entering into a deep, prolonged metabolic depression known as aestivation. Throughout aestivation, C. alboguttata is immobilised within a cast-like cocoon of shed skin and ceases feeding and moving. Remarkably, these frogs exhibit very little muscle atrophy despite extended disuse and fasting. The overall aim of the current research study was to gain a better understanding of the physiological, cellular and molecular basis underlying resistance to muscle disuse atrophy in C. alboguttata. The first aim of this study was to develop a genomic resource for C. alboguttata by sequencing and functionally characterising its skeletal muscle transcriptome, and to conduct gene expression profiling to identify transcriptional pathways associated with metabolic depression and maintenance of muscle function in aestivating burrowing frogs. A transcriptome was assembled using next-generation short read sequencing followed by a comparison of gene expression patterns between active and four-month aestivating C. alboguttata. This identified a complex suite of gene expression changes that occur in muscle during aestivation and provides evidence that aestivation in burrowing frogs involves transcriptional regulation of genes associated with cytoskeletal remodelling, avoidance of oxidative stress, energy metabolism, the cell stress response, cell death and survival and epigenetic modification. In particular, the expression levels of genes encoding cell cycle regulatory-, pro-survival and chromatin remodelling proteins, such as serine/threonine-protein kinase Chk1, cell division protein kinase 2, survivin, vesicular overexpressed in cancer prosurvival protein 1 and histone-binding protein RBBP4, were upregulated in aestivators. The second aim of this study was to examine the potential role of mitochondrial ROS in the regulation of muscle mass and function during aestivation in C. alboguttata. In mammals, muscle disuse atrophy has been associated with oxidative damage due to increased mitochondrial ROS production. C. alboguttata reduced skeletal muscle mitochondrial respiration by approximately 50% following four months of aestivation, while mitochondrial ROS production was more than 80% lower in aestivating skeletal muscle relative to controls when mitochondrial substrates were present at physiologically-relevant concentrations. In contrast to skeletal muscle, cardiac muscle of aestivating frogs must remain relatively active to still maintain adequate perfusion of organs. Aestivating frogs maintained cardiac mitochondrial respiration and ROS production at levels similar to those of control animals. Accelerated protein degradation in mammalian skeletal muscle has been linked to increased mitochondrial ROS production and oxidative stress. When ROS are in excess, a number of proteolytic pathways appear to play a pivotal role in the development of atrophy in inactive muscle fibres including the cytosolic calcium-dependent calpains. The aim of the final chapter was to determine if aestivating C. alboguttata are able to resist disuse-induced atrophy as a consequence of the downregulation of calpain proteases in skeletal muscle. The enzyme activity, protein abundance and gene expression levels of calpain isoforms were examined in skeletal muscle of aestivating and control C. alboguttata. There was no decrease in the protein abundances of calpain 1 or calpain 2 in aestivating C. alboguttata muscle relative to controls. Similarly, gene expression and enzyme activity levels of calpain 1 and 2 were unaffected by aestivation. The protein abundance of ‘muscle-specific’ calpain 3, which is consistently downregulated during atrophic conditions, was also examined in aestivating muscle. Western blotting indicated that calpain 3 may be autolysed (and hence activated) in skeletal muscle of both active and aestivating frogs. Results from the current study suggest that the relative inhibition of muscle atrophy in aestivating C. alboguttata is multifactorial in origin. ATP-dependent chromatin remodelling appears to be an important mechanism to actively regulate gene expression throughout aestivation, while elevated expression of anti-apoptotic genes is likely to be critical in preventing premature apoptotic muscle fibre degradation. In addition, decreased rates of skeletal muscle mitochondrial respiration during aestivation allows energy savings to be maximised. Low levels of hydrogen peroxide production suggests that ROS can be suppressed in immobilised skeletal muscles of aestivating frogs, which in combination with bolstering antioxidant defences may protect against potential oxidative stress and preserve skeletal muscle structure during aestivation and during arousal. While it is difficult to determine the specific function of calpain 3 in C. alboguttata muscle, the maintenance (rather than an increase) of pre-aestivation enzyme activity, protein and mRNA abundances of calpains is consistent with the protection of muscle against uncontrolled proteolysis throughout aestivation." @default.
• W847075346 created "2016-06-24" @default.
• W847075346 creator A5083538381 @default.
• W847075346 date "2015-06-03" @default.
• W847075346 modified "2023-09-23" @default.
• W847075346 title "Mechanisms underlying inhibition of muscle disuse atrophy during aestivation in the green-striped burrowing frog, Cyclorana alboguttata" @default.
• W847075346 cites W133152425 @default.
• W847075346 cites W138029989 @default.
• W847075346 cites W1530473805 @default.
• W847075346 cites W1538521820 @default.
• W847075346 cites W1554211606 @default.
• W847075346 cites W1558821075 @default.
• W847075346 cites W1572403702 @default.
• W847075346 cites W1599729465 @default.
• W847075346 cites W173605260 @default.
• W847075346 cites W1759824788 @default.
• W847075346 cites W1773902424 @default.
• W847075346 cites W1870982485 @default.
• W847075346 cites W1901168225 @default.
• W847075346 cites W1931442304 @default.
• W847075346 cites W1944638604 @default.
• W847075346 cites W1948153928 @default.
• W847075346 cites W1964578417 @default.
• W847075346 cites W1966543415 @default.
• W847075346 cites W1966822536 @default.
• W847075346 cites W1967021011 @default.
• W847075346 cites W1967661621 @default.
• W847075346 cites W1970452249 @default.
• W847075346 cites W1970565730 @default.
• W847075346 cites W1973175580 @default.
• W847075346 cites W1973479147 @default.
• W847075346 cites W1975043059 @default.
• W847075346 cites W1977713833 @default.
• W847075346 cites W1978227841 @default.
• W847075346 cites W1978726512 @default.
• W847075346 cites W1978881920 @default.
• W847075346 cites W1979540894 @default.
• W847075346 cites W1980638751 @default.
• W847075346 cites W1981006523 @default.
• W847075346 cites W1981432929 @default.
• W847075346 cites W1981830493 @default.
• W847075346 cites W1982422465 @default.
• W847075346 cites W1986179983 @default.
• W847075346 cites W1986373036 @default.
• W847075346 cites W1986475258 @default.
• W847075346 cites W1988744799 @default.
• W847075346 cites W1989033613 @default.
• W847075346 cites W1992544914 @default.
• W847075346 cites W1992936195 @default.
• W847075346 cites W1994963220 @default.
• W847075346 cites W1995565902 @default.
• W847075346 cites W1995806196 @default.
• W847075346 cites W1996580957 @default.
• W847075346 cites W1997009529 @default.
• W847075346 cites W1999494304 @default.
• W847075346 cites W1999933016 @default.
• W847075346 cites W2001124008 @default.
• W847075346 cites W2001610389 @default.
• W847075346 cites W2001610514 @default.
• W847075346 cites W2003162584 @default.
• W847075346 cites W2003393786 @default.
• W847075346 cites W2004922235 @default.
• W847075346 cites W2006246922 @default.
• W847075346 cites W2006986876 @default.
• W847075346 cites W2007081799 @default.
• W847075346 cites W2007665926 @default.
• W847075346 cites W2009834359 @default.
• W847075346 cites W2011024719 @default.
• W847075346 cites W2011414424 @default.
• W847075346 cites W2011583521 @default.
• W847075346 cites W2011729627 @default.
• W847075346 cites W2011894191 @default.
• W847075346 cites W2012329777 @default.
• W847075346 cites W2012794101 @default.
• W847075346 cites W2014582629 @default.
• W847075346 cites W2014905930 @default.
• W847075346 cites W2014982530 @default.
• W847075346 cites W2015282756 @default.
• W847075346 cites W2015859860 @default.
• W847075346 cites W2016333933 @default.
• W847075346 cites W2017594318 @default.
• W847075346 cites W2017673413 @default.
• W847075346 cites W2018101209 @default.
• W847075346 cites W2019661465 @default.
• W847075346 cites W2021080095 @default.
• W847075346 cites W2021829052 @default.
• W847075346 cites W2023470228 @default.
• W847075346 cites W2025013064 @default.
• W847075346 cites W2025916138 @default.
• W847075346 cites W2026389064 @default.
• W847075346 cites W2026965565 @default.
• W847075346 cites W2027552568 @default.
• W847075346 cites W2027775756 @default.
• W847075346 cites W2028026223 @default.
• W847075346 cites W2028463459 @default.
• W847075346 cites W2029842508 @default.
• W847075346 cites W2030054492 @default.
• W847075346 cites W2030172671 @default.
• W847075346 cites W2032730269 @default.
• W847075346 cites W2032867735 @default.

• next