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- W84753345 abstract "This chapter describes in vitro induction and assay of helper T cells using unprimed purified mouse cell populations. 15 × 106 nylon-wool-purified, cortisone-resistant thymocytes and 5 × 105 peritoneal exudate macrophages purified by glass adherence, scraping, and dead-cell removal are cocultured with carrier protein in Marbrook–Diener culture chambers. After 4 days, 5–10 × 105 viable cells (or supernatant in graded doses) are added with haptencarrier conjugate to cultures containing 15 × 106 spleen cells from mice primed with DNP–ficoll 3–4 months earlier. After an additional 4 days, triplicate cultures are assayed for high affinity DNP-specific plaque-forming cells (PFC) using a hapten inhibitable hemolytic plaque assay technique. The capacity of cells (or supernatant) from the helper cell culture to enhance the anti-DNP PFC response of the recipient spleen cell culture to DNP-carrier conjugates is the assay for helper cell induction. The induction of helper cells requires that the T-cells, but not macrophages, proliferate prior to the manifestation of helper cell function. Once generated, however, the active cell is functionally resistant to the anti-mitotic effect of mitomycin C." @default.
- W84753345 created "2016-06-24" @default.
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- W84753345 date "1977-01-01" @default.
- W84753345 modified "2023-09-27" @default.
- W84753345 title "IN VITRO INDUCTION AND ASSAY OF HELPER T-CELLS USING UNPRIMED PURIFIED MOUSE CELL POPULATIONS" @default.
- W84753345 cites W1984202994 @default.
- W84753345 doi "https://doi.org/10.1016/b978-0-12-458050-3.50104-1" @default.
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