Matches in SemOpenAlex for { <https://semopenalex.org/work/W848452420> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W848452420 endingPage "11" @default.
- W848452420 startingPage "6" @default.
- W848452420 abstract "3.3'-Dichlorobenzidine(DCB) has be shown carcinogenic in several animals, and the development of non-invasive biomonitoring method in workers exposed with it is a very important subject. DNA adduct is a good biomarker for biomonitoring about carcinogens exposure, and lymphocytes is a good non-invasive samples. So we studied to analyze metabolites in blood lymphocytes of female Sprague-Dawley rats exposed orally with DCB(20, 30, and 40 mg/kg wt.) for 3 weeks. For analysis of them, we isolated DNA adducts from blood lymphocytes by using the enzymes method in /sup 32/P-postlabeling, and measured them by using gas chromatography/mass spectrometry-selected ion monitoring(GC/MS-SIM). 4-aminobiphenyl and phenanthrene-d/sub 10/ were added as internal standard for blank sample. Standard metabolites of DCB were synthesized with using pyridine and acetic acid which were promoter and controller in acetylation of DCB. And they were used for calibration curve. Our results showed two kinds of metabolites in DNA adducts of blood lymphocytes. They were N-acetyl 3,3'-dichlorobenzidine(acDCB) and N,N'-diacetyl 3,3'-dichiorobenzidine(di-acDCB ). They were combined with DNA at the same time as an acetyl of it was removed. So we measured DCB and acDCB for two kinds of metabolites in DNA adducts of blood lymphocytes. Our results showed the levels of DCB were 1.46∼2.26 times more than that of acDCB. And also the levels of metabolites in 20, 30 and 40 mg/kg wt. were gradually increased with going days from 1st to 3rd week. They are 1.66, 1.38 and 0.90 times in total metabolites, 1.76, 1.49 and 1.02 times in DCB, and 1.51, 1.22 and 1.28 times in acDCB. In conclusion, the results of this study showed DCB exposed to rats formed DNA adduct in blood lymphocytes after acetylated to N-acetyl 3.3'-dichloro benzidine(acDCB) and N,N'-diacetyl 3,3'-dichlorobenzidine(di-acDCB), and they could be analyzed by us ing gas chromatography/mass spectrometry-selected ion monitoring(GC/MS-SIM)." @default.
- W848452420 created "2016-06-24" @default.
- W848452420 creator A5028000614 @default.
- W848452420 creator A5035231672 @default.
- W848452420 date "2002-01-01" @default.
- W848452420 modified "2023-09-26" @default.
- W848452420 title "A Study on 10 Metabolites Separated from DNA Adduce of Blood Lymphocytes in Rats Exposed Orally with 3,3-dichlorobenzidine(DCB) by GC/MS-SIM" @default.
- W848452420 hasPublicationYear "2002" @default.
- W848452420 type Work @default.
- W848452420 sameAs 848452420 @default.
- W848452420 citedByCount "0" @default.
- W848452420 crossrefType "journal-article" @default.
- W848452420 hasAuthorship W848452420A5028000614 @default.
- W848452420 hasAuthorship W848452420A5035231672 @default.
- W848452420 hasConcept C114246631 @default.
- W848452420 hasConcept C153911025 @default.
- W848452420 hasConcept C1621761 @default.
- W848452420 hasConcept C162356407 @default.
- W848452420 hasConcept C185592680 @default.
- W848452420 hasConcept C203014093 @default.
- W848452420 hasConcept C205345274 @default.
- W848452420 hasConcept C2776597655 @default.
- W848452420 hasConcept C2781247443 @default.
- W848452420 hasConcept C43617362 @default.
- W848452420 hasConcept C55493867 @default.
- W848452420 hasConcept C86803240 @default.
- W848452420 hasConcept C98274493 @default.
- W848452420 hasConceptScore W848452420C114246631 @default.
- W848452420 hasConceptScore W848452420C153911025 @default.
- W848452420 hasConceptScore W848452420C1621761 @default.
- W848452420 hasConceptScore W848452420C162356407 @default.
- W848452420 hasConceptScore W848452420C185592680 @default.
- W848452420 hasConceptScore W848452420C203014093 @default.
- W848452420 hasConceptScore W848452420C205345274 @default.
- W848452420 hasConceptScore W848452420C2776597655 @default.
- W848452420 hasConceptScore W848452420C2781247443 @default.
- W848452420 hasConceptScore W848452420C43617362 @default.
- W848452420 hasConceptScore W848452420C55493867 @default.
- W848452420 hasConceptScore W848452420C86803240 @default.
- W848452420 hasConceptScore W848452420C98274493 @default.
- W848452420 hasIssue "4" @default.
- W848452420 hasLocation W8484524201 @default.
- W848452420 hasOpenAccess W848452420 @default.
- W848452420 hasPrimaryLocation W8484524201 @default.
- W848452420 hasRelatedWork W1492360045 @default.
- W848452420 hasRelatedWork W1572623761 @default.
- W848452420 hasRelatedWork W1979177700 @default.
- W848452420 hasRelatedWork W1983058318 @default.
- W848452420 hasRelatedWork W1988189952 @default.
- W848452420 hasRelatedWork W2010131995 @default.
- W848452420 hasRelatedWork W2010812082 @default.
- W848452420 hasRelatedWork W2019518017 @default.
- W848452420 hasRelatedWork W2022111486 @default.
- W848452420 hasRelatedWork W2043936345 @default.
- W848452420 hasRelatedWork W2059799936 @default.
- W848452420 hasRelatedWork W2062154092 @default.
- W848452420 hasRelatedWork W2069873241 @default.
- W848452420 hasRelatedWork W2074896148 @default.
- W848452420 hasRelatedWork W2145883115 @default.
- W848452420 hasRelatedWork W2163633847 @default.
- W848452420 hasRelatedWork W2164576024 @default.
- W848452420 hasRelatedWork W2413189555 @default.
- W848452420 hasRelatedWork W2783781445 @default.
- W848452420 hasRelatedWork W2963027621 @default.
- W848452420 hasVolume "28" @default.
- W848452420 isParatext "false" @default.
- W848452420 isRetracted "false" @default.
- W848452420 magId "848452420" @default.
- W848452420 workType "article" @default.