Matches in SemOpenAlex for { <https://semopenalex.org/work/W84982043> ?p ?o ?g. }
- W84982043 abstract "Though the aetiology of transmissible spongiform encephalopathies (TSEs) remains uncertain, proteinase resistant prion protein (PrP-Sc), a converted form of the normal cellular prion protein (PrP-C), accumulates in the lysosome of cells of the nervous systems of animals with TSEs. In this study, clinical and epidemiological examinations of bovine spongiform encephalopathy (BSE) were conducted in Korea. During the investigated period, none of the cattle exhibited typical clinical signs of BSE, such as behavioral disturbances, high sensitivity, and abnormal locomotion. Immunohistochemical analysis and western immunoblotting were established to detect PrP-Sc in the brain tissue using monoclonal antibody (MAb) F89/160.1.5, produced by immunizing mice with a synthetic peptide which corresponds to bovine PrP residues 146-159, NH2-SRPLIHFGSDYEDRC-COOH. Although some BSE-like spongiform changes were observed in bovine brains randomly collected from Korean slaughterhouses from 1996 to 1999, no PrP-Sc was detected in those brains with the established immunohistochemistry and western immunoblotting assay. Also, no positive reaction was observed in bovine brains infected with rabies. These immunohistochemical and western immunoblotting methods using MAbs, specifically reactive with conserved epitopes on ruminant PrP, can be used for postmortem diagnosis of BSE. Further, the method can be applied to antemortem and the preclinical diagnosis of ovine scrapie by detecting PrP-Sc in lymphoid tissues, such as the tonsils, third eyelid or peripheral lymph nodes." @default.
- W84982043 created "2016-06-24" @default.
- W84982043 creator A5007771555 @default.
- W84982043 creator A5021812132 @default.
- W84982043 creator A5025152012 @default.
- W84982043 creator A5068199110 @default.
- W84982043 creator A5078002615 @default.
- W84982043 creator A5086969920 @default.
- W84982043 date "2001-01-01" @default.
- W84982043 modified "2023-09-24" @default.
- W84982043 title "Immunohistochemical detection of Prion protein (PrP-Sc) and epidemiological study of BSE in Korea" @default.
- W84982043 cites W1534728973 @default.
- W84982043 cites W1550397805 @default.
- W84982043 cites W1970340910 @default.
- W84982043 cites W1979059592 @default.
- W84982043 cites W1992084965 @default.
- W84982043 cites W1999225959 @default.
- W84982043 cites W1999758548 @default.
- W84982043 cites W2003289563 @default.
- W84982043 cites W2007242005 @default.
- W84982043 cites W2011035634 @default.
- W84982043 cites W2013679718 @default.
- W84982043 cites W2018407130 @default.
- W84982043 cites W2029904589 @default.
- W84982043 cites W2064024073 @default.
- W84982043 cites W2068356468 @default.
- W84982043 cites W2069460222 @default.
- W84982043 cites W2072244190 @default.
- W84982043 cites W2075176268 @default.
- W84982043 cites W2076847487 @default.
- W84982043 cites W2083061760 @default.
- W84982043 cites W2083921318 @default.
- W84982043 cites W2094337931 @default.
- W84982043 cites W2108078938 @default.
- W84982043 cites W2111154464 @default.
- W84982043 cites W2121614001 @default.
- W84982043 cites W2138498069 @default.
- W84982043 cites W2146568899 @default.
- W84982043 cites W2155913624 @default.
- W84982043 cites W2159437919 @default.
- W84982043 cites W2162594375 @default.
- W84982043 cites W2165902759 @default.
- W84982043 cites W2414308994 @default.
- W84982043 cites W2419542511 @default.
- W84982043 cites W2474569052 @default.
- W84982043 cites W2486592658 @default.
- W84982043 cites W6008596 @default.
- W84982043 doi "https://doi.org/10.4142/jvs.2001.2.1.25" @default.
- W84982043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14614290" @default.
- W84982043 hasPublicationYear "2001" @default.
- W84982043 type Work @default.
- W84982043 sameAs 84982043 @default.
- W84982043 citedByCount "6" @default.
- W84982043 countsByYear W849820432017 @default.
- W84982043 crossrefType "journal-article" @default.
- W84982043 hasAuthorship W84982043A5007771555 @default.
- W84982043 hasAuthorship W84982043A5021812132 @default.
- W84982043 hasAuthorship W84982043A5025152012 @default.
- W84982043 hasAuthorship W84982043A5068199110 @default.
- W84982043 hasAuthorship W84982043A5078002615 @default.
- W84982043 hasAuthorship W84982043A5086969920 @default.
- W84982043 hasBestOaLocation W849820431 @default.
- W84982043 hasConcept C142724271 @default.
- W84982043 hasConcept C159047783 @default.
- W84982043 hasConcept C159654299 @default.
- W84982043 hasConcept C181199279 @default.
- W84982043 hasConcept C195616568 @default.
- W84982043 hasConcept C203014093 @default.
- W84982043 hasConcept C204232928 @default.
- W84982043 hasConcept C2776735649 @default.
- W84982043 hasConcept C2776797465 @default.
- W84982043 hasConcept C2778147599 @default.
- W84982043 hasConcept C2778471508 @default.
- W84982043 hasConcept C2779134260 @default.
- W84982043 hasConcept C2781131914 @default.
- W84982043 hasConcept C3019804061 @default.
- W84982043 hasConcept C542903549 @default.
- W84982043 hasConcept C55493867 @default.
- W84982043 hasConcept C71924100 @default.
- W84982043 hasConcept C86803240 @default.
- W84982043 hasConceptScore W84982043C142724271 @default.
- W84982043 hasConceptScore W84982043C159047783 @default.
- W84982043 hasConceptScore W84982043C159654299 @default.
- W84982043 hasConceptScore W84982043C181199279 @default.
- W84982043 hasConceptScore W84982043C195616568 @default.
- W84982043 hasConceptScore W84982043C203014093 @default.
- W84982043 hasConceptScore W84982043C204232928 @default.
- W84982043 hasConceptScore W84982043C2776735649 @default.
- W84982043 hasConceptScore W84982043C2776797465 @default.
- W84982043 hasConceptScore W84982043C2778147599 @default.
- W84982043 hasConceptScore W84982043C2778471508 @default.
- W84982043 hasConceptScore W84982043C2779134260 @default.
- W84982043 hasConceptScore W84982043C2781131914 @default.
- W84982043 hasConceptScore W84982043C3019804061 @default.
- W84982043 hasConceptScore W84982043C542903549 @default.
- W84982043 hasConceptScore W84982043C55493867 @default.
- W84982043 hasConceptScore W84982043C71924100 @default.
- W84982043 hasConceptScore W84982043C86803240 @default.
- W84982043 hasLocation W849820431 @default.
- W84982043 hasLocation W849820432 @default.