Matches in SemOpenAlex for { <https://semopenalex.org/work/W850195762> ?p ?o ?g. }
- W850195762 endingPage "172" @default.
- W850195762 startingPage "1" @default.
- W850195762 abstract "Pituitary-dependent hypercortisolism (PDH) is a common endocrinopathy in dogs, caused by an ACTH secreting adenoma in the pituitary gland. Dogs with PDH can be treated with medication, radiation therapy or surgery. Although postoperative results are good, long-term recurrences do occur in around 25% of the dogs. The aims of this thesis were to analyze the long-term follow-up of dogs with PDH treated with transsphenoidal hypophysectomy over a 20-year period and to study the prognostic value of peri-operative measurement of plasma ACTH and cortisol concentrations in predicting recurrence of hypercortisolism. The aims also included the identification of possible prognostic pituitary tissue markers with immunohistochemistry and gene expression analysis, and the identification and characterization of stem cells in the normal canine pituitary gland.We showed that dogs with a larger pituitary have shorter survival and disease free periods after surgery, and that the pituitary size of patients at time of surgery significantly increased over the 20-year period. The evaluation of the individual peri-operative hormone curves of ACTH and cortisol of hypophysectomy patients provided valuable information about the risk of recurrence. However, it was not possible to identify an exact cut-off point to identify all recurrences with a single hormone measurement. Therefore, analysis of other clinical parameters, like pituitary size, remains essential. The second part of the thesis focused on the expression of possible prognostic markers in pituitary tissue. We found that the expression of Ki-67 and PCNA (proliferation markers) were not significantly different between enlarged and non-enlarged pituitaries. The cell-cycle inhibitor p27kip1 trended to be expressed less in enlarged pituitaries compared to the non-enlarged pituitaries. We found Pax7 [removed]specific marker for intermediate lobe cells) in adenomas originating in the anterior and intermediate lobe, but we found no correlation between Pax7 expression or activation of the Pax7 signaling pathway to clinical parameters and outcome. In the process, also pituitary tissue specific reference genes were established.Isolation of stem cells from pituitary gland tissue is complicated by the lack of specific stem cell markers. We were not able to relate Sox2 [removed]possible pituitary stem cell marker) to clinical parameters in dogs with pituitary adenomas. Fluorescence activated cell sorting (FACS) was used to sort a side population (SP) of cells. FACS was previously used successfully in multiple tissue types to isolate a SP containing stem cells. In this thesis, we successfully isolated and characterized the SP-cells from six healthy canine pituitary glands.In conclusion, transsphenoidal hypophysectomy is an effective treatment for dogs with PDH. It remains unclear why, after initial remission, some dogs develop recurrence of hypercortisolism, whereas others do not. Of the markers studied in this thesis, p27kip1 and Pax7 remain interesting for future studies, indicating a disruption of cell-cycle control as the basis for pituitary tumorigenesis. However, these markers cannot be used as absolute prognostic markers. Continued studies are needed to unravel the role of stem cells in the pathogenesis of canine pituitary adenomas. What is urgently needed is a specific biomarker to predict surgical outcome, i.e. remission or recurrence." @default.
- W850195762 created "2016-06-24" @default.
- W850195762 creator A5085418184 @default.
- W850195762 date "2015-06-02" @default.
- W850195762 modified "2023-09-24" @default.
- W850195762 title "Prognostic factors for outcome of pituitary surgery in dogs with corticotroph adenomas" @default.
- W850195762 cites W156663091 @default.
- W850195762 cites W1649533596 @default.
- W850195762 cites W1974208182 @default.
- W850195762 cites W1987378831 @default.
- W850195762 cites W1991135730 @default.
- W850195762 cites W2005236094 @default.
- W850195762 cites W2009022446 @default.
- W850195762 cites W2011722112 @default.
- W850195762 cites W2018988068 @default.
- W850195762 cites W2020606253 @default.
- W850195762 cites W2020899639 @default.
- W850195762 cites W2024381232 @default.
- W850195762 cites W2027197718 @default.
- W850195762 cites W2030604268 @default.
- W850195762 cites W2044937492 @default.
- W850195762 cites W2045475945 @default.
- W850195762 cites W2048640907 @default.
- W850195762 cites W2052736372 @default.
- W850195762 cites W2055879869 @default.
- W850195762 cites W2068528952 @default.
- W850195762 cites W2076026993 @default.
- W850195762 cites W2083589718 @default.
- W850195762 cites W2090364077 @default.
- W850195762 cites W2092792707 @default.
- W850195762 cites W2094718142 @default.
- W850195762 cites W2115679080 @default.
- W850195762 cites W2126486896 @default.
- W850195762 cites W2128453336 @default.
- W850195762 cites W2131076971 @default.
- W850195762 cites W2133027863 @default.
- W850195762 cites W2152849156 @default.
- W850195762 cites W2160677477 @default.
- W850195762 cites W2161244866 @default.
- W850195762 cites W2163733063 @default.
- W850195762 cites W2164654891 @default.
- W850195762 cites W2166722271 @default.
- W850195762 cites W2167021776 @default.
- W850195762 cites W2168404575 @default.
- W850195762 cites W2170613693 @default.
- W850195762 cites W2313061021 @default.
- W850195762 cites W49649439 @default.
- W850195762 hasPublicationYear "2015" @default.
- W850195762 type Work @default.
- W850195762 sameAs 850195762 @default.
- W850195762 citedByCount "0" @default.
- W850195762 crossrefType "dissertation" @default.
- W850195762 hasAuthorship W850195762A5085418184 @default.
- W850195762 hasConcept C126322002 @default.
- W850195762 hasConcept C134018914 @default.
- W850195762 hasConcept C142724271 @default.
- W850195762 hasConcept C204232928 @default.
- W850195762 hasConcept C2776810848 @default.
- W850195762 hasConcept C2777428134 @default.
- W850195762 hasConcept C2777658017 @default.
- W850195762 hasConcept C2779134260 @default.
- W850195762 hasConcept C2779318953 @default.
- W850195762 hasConcept C2780104272 @default.
- W850195762 hasConcept C2780546910 @default.
- W850195762 hasConcept C2781449511 @default.
- W850195762 hasConcept C71315377 @default.
- W850195762 hasConcept C71924100 @default.
- W850195762 hasConcept C79381400 @default.
- W850195762 hasConceptScore W850195762C126322002 @default.
- W850195762 hasConceptScore W850195762C134018914 @default.
- W850195762 hasConceptScore W850195762C142724271 @default.
- W850195762 hasConceptScore W850195762C204232928 @default.
- W850195762 hasConceptScore W850195762C2776810848 @default.
- W850195762 hasConceptScore W850195762C2777428134 @default.
- W850195762 hasConceptScore W850195762C2777658017 @default.
- W850195762 hasConceptScore W850195762C2779134260 @default.
- W850195762 hasConceptScore W850195762C2779318953 @default.
- W850195762 hasConceptScore W850195762C2780104272 @default.
- W850195762 hasConceptScore W850195762C2780546910 @default.
- W850195762 hasConceptScore W850195762C2781449511 @default.
- W850195762 hasConceptScore W850195762C71315377 @default.
- W850195762 hasConceptScore W850195762C71924100 @default.
- W850195762 hasConceptScore W850195762C79381400 @default.
- W850195762 hasLocation W8501957621 @default.
- W850195762 hasOpenAccess W850195762 @default.
- W850195762 hasPrimaryLocation W8501957621 @default.
- W850195762 hasRelatedWork W1966184376 @default.
- W850195762 hasRelatedWork W1987624782 @default.
- W850195762 hasRelatedWork W2166867598 @default.
- W850195762 hasRelatedWork W2354182433 @default.
- W850195762 hasRelatedWork W2359805333 @default.
- W850195762 hasRelatedWork W2373017038 @default.
- W850195762 hasRelatedWork W2382766772 @default.
- W850195762 hasRelatedWork W2385433336 @default.
- W850195762 hasRelatedWork W2388777439 @default.
- W850195762 hasRelatedWork W2391509259 @default.
- W850195762 hasRelatedWork W2392108523 @default.
- W850195762 hasRelatedWork W2414141880 @default.