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- W850320420 abstract "ABSTRACTThe ability to detect and respond to environmental signals is fundamental in coordinating floralinduction in plants to favourable conditions. An important flowering time cue is day length andit is proposed that light signals are perceived and measured by an interaction betweenphotoreceptors and an internal pacemaker, the circadian clock. The control of flowering hasbeen best characterised in the model plant Arabidopsis thaliana L. Heynh (Arabidopsis). TheGIGANTEA (GI) gene has a complex role in both the promotion of flowering in response tophotoperiod and the regulation of the circadian clock. The expression of GI is under circadiancontrol and is affected by day length, light quality and temperature changes. The GI protein isalso circadian regulated and is actively degraded in the dark.The biochemical function of GI is unknown and one method to elucidate the role of this proteinis to identify protein interactors. The aim of this thesis project was to characterise proteins thatinteracted with GI. Previously, the BELL-LIKE HOMEODOMAIN 3 (BLH3) protein wasidentified as a putative GI protein interactor. As part of this thesis work, yeast 2-hybrid and invitro pull down assays were utilised to confirm the interaction between GI and BLH3. Sequenceand phylogenetic analyses were used to further examine the BELL family of proteins. TheBELL-LIKE HOMEODOMAIN 10 (BLH10) protein was found to be closely related to BLH3and also interacted with GI. Reverse 2-hybrid assays were used to determine the regions ordomains within the GI, BLH3 and BLH10 proteins required to mediate protein interactions.Expression assays established that the BLH3 and BLH10 transcripts were present throughoutplant tissues and times of development. Further analyses revealed that BLH3 and BLH10 are notdirectly regulated by the circadian clock. The results of GFP expression assays demonstratedthat the BLH3 protein is localised to the nucleus in plant cells. Transgenic blh3 and blh10mutant plants were identified and analysed for flowering and light response phenotypes. BLH3and BLH10 do not function with GI in the photoperiodic pathway to control flowering, yet theblh3 and blh10 mutants do have a flowering phenotype in short day conditions. Like gi, the blh3and blh10 mutants exhibited exaggerated hypocotyl elongation in response to red and low lightconditions. These results are suggestive of a role for BLH3, BLH10 and GI in flowering and deetiolationresponses to specific light conditions in plants." @default.
- W850320420 created "2016-06-24" @default.
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- W850320420 date "2006-01-01" @default.
- W850320420 modified "2023-09-23" @default.
- W850320420 title "Characterising GIGANTEA interactors: the BELL-LIKE HOMEODOMAIN 3 and BELL-LIKE HOMEODOMAIN 10 proteins" @default.
- W850320420 hasPublicationYear "2006" @default.
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